[The anti-athetotic effects of heme-L-lysinate in a rabbit model of atherosclerosis].

Abstract

OBJECTIVE:

To investigate the anti-atherotic effects of heme-L-lysinate in a rabbit model of atherosclerosis and related machanisms.

METHODS:

Adult rabbits were treated with 1% cholesterol diet (chol group, n = 8) or 1% cholesterol diet plus heme-L-lysinate (9 mgxkg(-1)xd(-1), Heme group, n = 8) or 1% cholesterol diet plus isotonic Na chloride (Na chloride group, n = 8) for 10 weeks. Eight rabbits fed with normal diet served as normal control. Aortic carbon monoxide (CO) was quantified spectrophotometrically by the formation of carboxyhaemoglobin (HbCO). Aortic heme oxygenase-1 (HO-1) and HSP70 mRNA and protein expressions were detected by RT-PCR and immunohistochemical staining.

RESULTS:

Aortic CO production and HO-1 activity were significantly increased in chol group and Na chloride group compared those in normal control group (P < 0.01). Aortic plaque area was significantly reduced in heme group (26.6% +/- 9.2%) than that in chol group (42.3% +/- 8.7%, P < 0.01). Aortic HO-1 expression, CO production and HSP70 were significantly increased in heme group than those in chol group and Na chloride group (all P < 0.01).

CONCLUSIONS:

Heme-L-lysinate could attenuate atherosclerotic progression through upregulating HO-1 and HSP70 expression and increasing CO production in this model.

• Authors: Xiao-bing Wang, Wan-lin Wei
• Journal: Zhonghua xin xue guan bing za zhi (Zhonghua Xin Xue Guan Bing Za Zhi) Vol. 38 Issue 5 Pg. 450-4 (May 2010) ISSN: 0253-3758 [Print] China
• PMID: 20654107 (Publication Type: Journal Article)

Chemical References

• Cholesterol, Dietary
• HSC70 Heat-Shock Proteins
• RNA, Messenger
• heme lysinate
• Heme
• Carbon Monoxide
• Heme Oxygenase-1
• Lysine

Topics

• Animals
• Atherosclerosis (metabolism, prevention & control)
• Carbon Monoxide (metabolism)
• Cholesterol, Dietary
• Diet, Atherogenic
• Disease Models, Animal
• HSC70 Heat-Shock Proteins (genetics, metabolism)
• Heme (analogs & derivatives, pharmacology)
• Heme Oxygenase-1 (metabolism)
• Lysine (analogs & derivatives, pharmacology)
• Male
• RNA, Messenger (genetics)
• Rabbits