Abstract | OBJECTIVE: METHODS: The hepatitis B surface antigen ( HBsAg) was prepared by the genetic engineering technique, BCG was produced using routine biological technique, and then the finished products of the HB-BCG combined vaccine were processed on the above foundation. The content of HBsAg was measured by Enzyme linked immunosorbent assay (ELISA), the immune effect of BCG was detected by purified protein derivative ( PPD) test. Cellular immune response, safety, partial poison and allergy were tested. The stability of HB-BCG combined vaccine was detected by ELISA and viable count method. RESULTS: CONCLUSION: The immune effects of the HB-BCG combined vaccine were not lower than the two kinds of single dose vaccine, it had good safety.
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Authors | Lijie Jin, Guangyi Wang, Xiaolin Zhao, Zhi Wang, Lihua Yang, Aidong Yu, Yanling Xu, Wei Li |
Journal | Vaccine
(Vaccine)
Vol. 28
Issue 37
Pg. 6041-51
(Aug 23 2010)
ISSN: 1873-2518 [Electronic] Netherlands |
PMID | 20619374
(Publication Type: Comparative Study, Journal Article)
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Copyright | Copyright 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- BCG Vaccine
- Cytokines
- Hepatitis B Antibodies
- Hepatitis B Surface Antigens
- Hepatitis B Vaccines
- Vaccines, Combined
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Topics |
- Animals
- BCG Vaccine
(adverse effects, immunology)
- Cytokines
(blood)
- Enzyme-Linked Immunosorbent Assay
- Female
- Guinea Pigs
- Hepatitis B
(prevention & control)
- Hepatitis B Antibodies
(blood)
- Hepatitis B Surface Antigens
(immunology)
- Hepatitis B Vaccines
(adverse effects, immunology)
- Immunity, Cellular
- Immunity, Humoral
- Immunization, Secondary
- Lymphocyte Activation
- Male
- Mice
- Mice, Inbred BALB C
- T-Lymphocytes
(immunology)
- Toxicity Tests
- Vaccines, Combined
(immunology)
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