Pharmacokinetic and biodistribution studies of N-(2-hydroxypropyl)methacrylamide copolymer-dexamethasone conjugates in adjuvant-induced arthritis rat model.

Abstract

N-(2-Hydroxypropyl)methacrylamide (HPMA) copolymer has been found to be arthrotropic (joint-targeting) in the adjuvant-induced arthritis (AA) rat model using magnetic resonance imaging (MRI). In this manuscript, we report the quantitative pharmacokinetics and biodistribution (PK/BD) of (125)I-labeled HPMA copolymer-dexamethasone conjugate (P-Dex) in AA rats. Structural parameters of the prodrug such as the molecular weight (MW) and Dex content were found to have strong impact on the PK/BD profiles of P-Dex. The increase of MW (14,000, 24,000, and 42,000 g/mol) and Dex content (0, 151, and 313 micromol/g) enhances the arthrotropism of P-Dex. For the conjugate with highest MW and Dex content (P-H-M(W)/Dex), the percentage of injected doses per gram (ID/g) of ankle synovial tissue at day seventh postadministration is 1% g(-1), which confirms P-Dex as an arthrotropic macromolecular prodrug. For liver and spleen, the ID/g values are 0.51 and 3.64% g(-1), respectively. As an antigen-presenting organ, the sequestration of the prodrug by spleen may be explained by its abnormal enlargement associated with the systemic inflammatory disease model. Gradual reduction of spleen weight due to the inflammation resolution effect of P-Dex may also contribute to the high ID/g values. Increase of Dex content and reduction of MW would increase P-Dex distribution to kidney. The highest ID/g value for kidney at day seventh postadministration (0.91% g(-1)) was found with P-L-M(w) (MW = 14,000 g/mol, Dex content =288 micromol/g), which may suggest kidney tubuli reabsorption of the conjugates. The P-Dex's distribution to heart and lung is minimum.

• Authors: Ling-Dong Quan, Fang Yuan, Xin-Ming Liu, Jian-Geng Huang, Yazen Alnouti, Dong Wang
• Journal: Molecular pharmaceutics (Mol Pharm) Vol. 7 Issue 4 Pg. 1041-9 (Aug 02 2010) ISSN: 1543-8392 [Electronic] United States
• PMID: 20557133 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Acrylamides
• Anti-Inflammatory Agents
• Polymers
• Dexamethasone
• N-(2-hydroxypropyl)methacrylamide

Topics

• Acrylamides (chemistry)
• Animals
• Anti-Inflammatory Agents (chemistry, pharmacokinetics, therapeutic use)
• Arthritis, Experimental (blood, drug therapy)
• Dexamethasone (chemistry, pharmacokinetics, therapeutic use)
• Male
• Polymers (chemical synthesis, chemistry, pharmacokinetics)
• Rats