Potent lentiviral restriction by a synthetic feline TRIM5 cyclophilin A fusion.

Abstract

A synthetic feline TRIM5-cyclophilin A fusion protein (feTRIMCyp) was generated and transduced into feline cells. feTRIMCyp was highly efficient at preventing infection with human (HIV) and feline (FIV) immunodeficiency virus pseudotypes, and feTRIMCyp-expressing cells resisted productive infection with either FIV-Fca or FIV-Pco. The restriction of FIV infection by feTRIMCyp was reversed by the cyclosporine (Cs) derivatives NIM811 and Debio-025 but less so by Cs itself. FeTRIMCyp and FIV infections of the cat offer a unique opportunity to evaluate TRIMCyp-based approaches to genetic therapy for HIV infection and the treatment of AIDS.

• Authors: Isabelle Dietrich, Angela Macintyre, Elizabeth McMonagle, Amanda J Price, Leo C James, William A McEwan, Margaret J Hosie, Brian J Willett
• Journal: Journal of virology (J Virol) Vol. 84 Issue 17 Pg. 8980-5 (Sep 2010) ISSN: 1098-5514 [Electronic] United States
• PMID: 20554781 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Restriction Factors
• Carrier Proteins
• Recombinant Fusion Proteins
• Tripartite Motif Proteins
• TRIM5 protein, human
• Ubiquitin-Protein Ligases
• Cyclophilin A

Topics

• Animals
• Antiviral Restriction Factors
• Carrier Proteins (chemical synthesis, genetics, metabolism)
• Cats
• Cell Line
• Cyclophilin A (chemical synthesis, genetics, metabolism)
• Disease Models, Animal
• Feline Acquired Immunodeficiency Syndrome (prevention & control, virology)
• HIV Infections (prevention & control, virology)
• HIV-1 (physiology)
• Humans
• Immunodeficiency Virus, Feline (physiology)
• Recombinant Fusion Proteins (chemical synthesis, genetics, metabolism)
• Tripartite Motif Proteins
• Ubiquitin-Protein Ligases
• Virus Internalization