Abstract |
Gene regulation in trypanosomatids occurs mainly by post-transcriptional mechanisms modulating mRNA stability and translation. We have investigated heat shock protein (HSP) 70 gene regulation in Trypanosoma cruzi, the causal agent of Chagas' disease. The HSP70 mRNA's half-life increases after heat shock, and the stabilization is dependent on protein synthesis. In a cell-free RNA decay assay, a U-rich region in the 3' untranslated region (UTR) is a target for degradation, which is reduced when in the presence of protein extracts from heat shocked cells. In a transfected reporter gene assay, both the 5'- and 3'-UTRs confer temperature-dependent regulation. Both UTRs must be present to increase mRNA stability at 37 degrees C, indicating that the 5'- and 3'-UTRs act cooperatively to stabilize HSP70 mRNA during heat shock. We conclude that HSP70 5'- and 3'-UTRs regulate mRNA stability during heat shock in T. cruzi.
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Authors | Deivid C Rodrigues, Rosane Silva, Edson Rondinelli, Turán P Urményi |
Journal | Experimental parasitology
(Exp Parasitol)
Vol. 126
Issue 2
Pg. 245-53
(Oct 2010)
ISSN: 1090-2449 [Electronic] United States |
PMID | 20493845
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- 3' Untranslated Regions
- 5' Untranslated Regions
- DNA, Protozoan
- HSP70 Heat-Shock Proteins
- RNA, Messenger
- Chloramphenicol O-Acetyltransferase
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Topics |
- 3' Untranslated Regions
(genetics, physiology)
- 5' Untranslated Regions
(genetics, physiology)
- Base Sequence
- Blotting, Northern
- Chloramphenicol O-Acetyltransferase
(genetics, metabolism)
- DNA, Protozoan
(chemistry, genetics, metabolism)
- Gene Expression Regulation
(physiology)
- HSP70 Heat-Shock Proteins
(genetics, metabolism)
- Half-Life
- Hot Temperature
- Plasmids
(genetics, metabolism)
- RNA, Messenger
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Transfection
- Trypanosoma cruzi
(genetics, metabolism)
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