Mevalonate kinase deficiency (MKD) is a rare inborn auto-inflammatory disease due to the impairment of the pathway for the biosynthesis of
cholesterol and non-
sterol isoprenoids. The shortage of
isoprenoids compounds and in particular of geranylgeranylpyrophosphate (GGPP) was recently associated to the MKD characteristic inflammatory attacks. The aim of this study is to demonstrate that the normalization of the
mevalonate pathway intermediates levels and in particular of GGPP, through the specific inhibition of farnesyl-
transferase (FT) with
Manumycin A could ameliorate the inflammatory phenotype of MKD patients. The effect of
Manumycin A was first evaluated in MKD mouse and cellular models, chemically obtained using the aminobisphosphonate
alendronate (ALD), and then in monocytes isolated from 2 MKD patients. Our findings were compared to those obtained by using natural exogenous
isoprenoids (NEIs).
Manumycin A was able to significantly reduce the inflammatory
marker serum amyloid A in ALD-treated Balb/c mice, as well as
IL-1 beta secretion in ALD-monocytes and in MKD patients. These results clearly showed that, through the inhibition of FT, an increased number of
mevalonate pathway intermediates could be redirected towards the synthesis of GGPP diminishing the inflammatory response. The importance in limiting the shortage of GGPP was emphasized by the anti-inflammatory effect of NEIs that, due to their biochemical structure, can enter the MKD pathway. In conclusion,
manumycin A, as well as NEIs, showed anti-inflammatory effect in MKD models and especially in MKD-monocytes, suggesting novel approaches in the treatment of MKD, an
orphan disease without any efficacious treatment currently available.