Binding of functionalized paramagnetic nanoparticles to bacterial lipopolysaccharides and DNA.

Abstract

Magnetite and metallic cobalt-based nanoparticles with sizes ranging from 10 to 300 nm and surface-functionalized with poly(hexamethylene biguanide) (PHMBG) are introduced as capable lipopolysaccharide (LPS)-sequestering agents. The nanoparticles efficiently bind to whole E. coli cells and can be used to separate the cells effectively from suspension using a magnet. A fluorescence dye displacement assay shows strong affinities of the nanoparticles for lipid A, the glycolipid component of LPS responsible for septic shock. The particle-lipid A affinity is of the same order of magnitude or higher than that of polymyxin B. The affinity of smaller (< 50 nm) magnetite particles modified with PHMBG to lipid A is several-fold higher than that of their larger counterparts (> 100 nm) due to their higher surface area to volume ratio. The nanoparticles possess high saturation capacity for double-stranded lambdaDNA from E. coli, with which particle-polyelectrolyte complexes are formed. The PHMBG-modified nanoparticles are potent bactericides, inhibiting E. coli viability and growth at concentrations at < or = 10 microg/mL.

• Authors: Lev Bromberg, Emily P Chang, Carmen Alvarez-Lorenzo, Beatriz Magariños, Angel Concheiro, T Alan Hatton
• Journal: Langmuir : the ACS journal of surfaces and colloids (Langmuir) Vol. 26 Issue 11 Pg. 8829-35 (Jun 01 2010) ISSN: 1520-5827 [Electronic] United States
• PMID: 20155986 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Fluorescent Dyes
• Lipopolysaccharides
• DNA

Topics

• DNA (chemistry)
• Escherichia coli (chemistry, growth & development)
• Fluorescent Dyes (chemistry)
• Lipopolysaccharides (chemistry)
• Nanoparticles
• Spectrometry, Fluorescence