Abstract | CONTEXT: OBJECTIVE: We tested antiangiogenic and antiproliferative efficacy of tetrac and tetrac nanoparticles ( tetrac NP) against human medullary thyroid carcinoma (h-MTC) implants in the chick chorioallantoic membrane (CAM) and h-MTC xenografts in the nude mouse. DESIGN: h-MTC cells were implanted in the CAM model (n = 8 per group); effects of tetrac and tetrac NP at 1 microg/CAM were determined on tumor angiogenesis and tumor growth after 8 d. h-MTC cells were also implanted sc in nude mice (n = 6 animals per group), and actions on established tumor growth of unmodified tetrac and tetrac NP ip were determined. RESULTS: In the CAM, tetrac and tetrac NP inhibited tumor growth and tumor-associated angiogenesis. In the nude mouse xenograft model, established 450-500 mm(3) h-MTC tumors were reduced in size over 21 d by both tetrac formulations to less than the initial cell mass (100 mm(3)). Tumor tissue hemoglobin content of xenografts decreased by 66% over the course of administration of each drug. RNA microarray and quantitative real-time PCR of tumor cell mRNAs revealed that both tetrac formulations significantly induced antiangiogenic thrombospondin 1 and apoptosis activator gene expression. CONCLUSIONS:
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Authors | M Yalcin, E Dyskin, L Lansing, D J Bharali, S S Mousa, A Bridoux, A H Hercbergs, H Y Lin, F B Davis, G V Glinsky, A Glinskii, J Ma, P J Davis, S A Mousa |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 95
Issue 4
Pg. 1972-80
(Apr 2010)
ISSN: 1945-7197 [Electronic] United States |
PMID | 20133461
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Excipients
- Hemoglobins
- RNA, Neoplasm
- Polylactic Acid-Polyglycolic Acid Copolymer
- Polyglycolic Acid
- Lactic Acid
- tetraiodothyroacetic acid
- Thyroxine
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Topics |
- Animals
- Antineoplastic Agents
- Body Weight
(drug effects)
- Carcinoma, Medullary
(drug therapy, pathology)
- Cells, Cultured
- Chick Embryo
- Chorioallantoic Membrane
(pathology)
- Excipients
- Female
- Hemoglobins
(metabolism)
- Humans
- Lactic Acid
- Mice
- Mice, Nude
- Nanoparticles
- Neovascularization, Pathologic
(pathology, prevention & control)
- Oligonucleotide Array Sequence Analysis
- Polyglycolic Acid
- Polylactic Acid-Polyglycolic Acid Copolymer
- RNA, Neoplasm
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Thyroid Neoplasms
(drug therapy, pathology)
- Thyroxine
(administration & dosage, analogs & derivatives, therapeutic use)
- Xenograft Model Antitumor Assays
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