[Analysis on mutation of S gene and P gene of hepatitis B virus in two counties of Sichuan Province].

Abstract

OBJECTIVE:

To analyze HBV S gene/P gene mutation in 2 counties (districts) of Sichuan province.

METHODS:

HBV DNA were extracted from sera positive both for HBsAg and HBeAg. After PCR and nucleotide sequencing, nucleotide/amino acid mutation in S and P gene were compared and analyzed.

RESULTS:

Of 47 serum samples from patients with chronic HBV infection, amino acid mutation in 'a' determinant occurred in 12 samples (25.53%,12/47), correlating with T126A, I126T/S, P127T, T131N, M133L, M133T and T140I; high proportion of mutation clustered in first loop of 'a' determinant (92.86%,13/14), rtV207I mutation in C domain of reverse transcription occured in one sample.

CONCLUSION:

Naturally occurring mutation in 'a' determinant clustered predominantly in the first loop and usually associated with altered antigenicity, posing a potential threat to successfully vaccinated individuals; Lamivudine-resistant mutant might occur in patient even without nucleotide analogue treatment.

• Authors: Wen-Bin Tong, Ji-Lan He, Li Sun
• Journal: Zhongguo yi miao he mian yi (Zhongguo Yi Miao He Mian Yi) Vol. 15 Issue 1 Pg. 45-8 (Feb 2009) ISSN: 1006-916X [Print] China
• PMID: 20077675 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Gene Products, pol
• Hepatitis B Surface Antigens
• P protein, Hepatitis B virus

Topics

• Amino Acid Sequence
• China
• Gene Products, pol (chemistry, genetics)
• Hepatitis B (virology)
• Hepatitis B Surface Antigens (chemistry, genetics)
• Hepatitis B virus (chemistry, genetics, isolation & purification)
• Humans
• Molecular Sequence Data
• Mutation
• Sequence Alignment