Abstract |
Cigarette smoking condensate (CSC) contains oxidant compounds able to generate superoxide. The aim of the present study was to investigate the effect of the exposure to CSC on: (1) free radical production, (2) the gene expression of the antioxidant enzymes Cu-Zn superoxide dismutase (SOD1), Mn superoxide dismutase (SOD2), Glutathione Peroxidase (GPx), and catalase (CAT), and (3) cell survival in human neuroblastoma SH-SY5Y cells. The results showed that exposure (24 h) to different concentrations (10-150 μg/ml) of CSC caused a dose dependent cell injury that was coupled to the maximal increase of free radical production. These events were prevented by the addition to the incubation medium of the scavenger Vitamin E (50 μM). Furthermore, CSC exposure caused a reduction of the gene expression of the antioxidant enzymes SOD1, SOD2, GPx, and CAT that was counteracted by Vitamin E (50 μM). These results suggest that CSC exposure can induce a free radical overcharge that may be responsible for the inhibition of antioxidant enzymes expression and cell injury in SH-SY5Y human neuroblastoma cells. In fact the scavenger vitamin E can block both cell injury and inhibition of SOD1, SOD2, GPx, and CAT induced by CSC exposure.
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Authors | Michela Russo, Stefania Cocco, Agnese Secondo, Annagrazia Adornetto, Antonella Bassi, Alfredo Nunziata, Giuliano Polichetti, Bruna De Felice, Simona Damiano, Rosalba Serù, Paolo Mondola, Gianfranco Di Renzo |
Journal | Neurotoxicity research
(Neurotox Res)
Vol. 19
Issue 1
Pg. 49-54
(Jan 2011)
ISSN: 1476-3524 [Electronic] United States |
PMID | 19949914
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Free Radicals
- Particulate Matter
- SOD1 protein, human
- Smoke
- Vitamin E
- Catalase
- Glutathione Peroxidase
- Superoxide Dismutase
- Superoxide Dismutase-1
- superoxide dismutase 2
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Topics |
- Catalase
(antagonists & inhibitors, biosynthesis)
- Cell Line, Tumor
- Free Radicals
(metabolism)
- Gene Expression Regulation, Enzymologic
(drug effects, physiology)
- Glutathione Peroxidase
(antagonists & inhibitors, biosynthesis)
- Humans
- Neuroblastoma
(enzymology, metabolism, pathology)
- Particulate Matter
(toxicity)
- Smoke
(adverse effects)
- Superoxide Dismutase
(antagonists & inhibitors, biosynthesis)
- Superoxide Dismutase-1
- Nicotiana
(adverse effects)
- Vitamin E
(pharmacology)
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