Effect of hirulog-like peptide on balloon catheter injury-induced neointimal formation in femoral arteries of minipigs and relationship with inflammatory mediators.

Abstract

Vascular intervention-induced neointimal formation is a major drawback for managing atherosclerotic cardiovascular diseases using invasive vascular procedures. Our previous studies demonstrated that hirulog-like peptide (HLP) reduced balloon catheter dilation-induced neointimal formation or restenosis in carotid arteries of rats or atherosclerotic rabbits with less interruption in coagulation or bleeding than heparin or hirulog-1. The present study examined the effect of HLP on balloon catheter injury-induced neointimal formation in femoral arteries of minipigs. Intravenous infusion of HLP (1.6 mg/kg/h for 4 h started 0.5 h before the intervention) or unfractured heparin (50 U/kg/h for 4 h) significantly reduced neointimal formation in femoral arteries 4 weeks after intervention compared with the vehicle. Heparin, but not HLP, significantly prolonged activated partial thromboplastin time. HLP or heparin significantly reduced vascular intervention-induced increases in C-reactive protein, P-selectin and interleukin-6 in serum. HLP, but not heparin, normalized vascular injury-induced increase in P-selectin in platelets. The results of the present study suggest that HLP is an effective agent for preventing balloon catheter injury-induced neointimal formation in femoral arteries of minipigs. The beneficial effects of HLP on vascular injury-induced neointimal formation may partially result from its inhibition on inflammatory mediators.

• Authors: Xiao-Feng Tang, Jian-Jun Liu, Yong-Jie Wu, Ke-Ming Chen, Yong Jin, Ping-Jin Gao, Ding-Liang Zhu, Garry X Shen
• Journal: Journal of vascular research (J Vasc Res) Vol. 47 Issue 3 Pg. 262-9 ( 2010) ISSN: 1423-0135 [Electronic] Switzerland
• PMID: 19907188 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 S. Karger AG, Basel.

Chemical References

• Anti-Inflammatory Agents
• Fibrinolytic Agents
• Hirudins
• Inflammation Mediators
• Interleukin-6
• P-Selectin
• Peptide Fragments
• Recombinant Proteins
• Heparin
• C-Reactive Protein
• bivalirudin

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage, pharmacology)
• Blood Coagulation (drug effects)
• C-Reactive Protein (metabolism)
• Catheterization (adverse effects)
• Femoral Artery (drug effects, immunology, injuries, pathology)
• Fibrinolytic Agents (administration & dosage, pharmacology)
• Heparin (pharmacology)
• Hirudins (administration & dosage, pharmacology)
• Inflammation Mediators (blood)
• Infusion Pumps
• Infusions, Intravenous
• Interleukin-6 (blood)
• Male
• Models, Animal
• P-Selectin (blood)
• Peptide Fragments (administration & dosage, pharmacology)
• Platelet Aggregation (drug effects)
• Recombinant Proteins (administration & dosage, pharmacology)
• Swine
• Swine, Miniature
• Time Factors
• Tunica Intima (drug effects, immunology, injuries, pathology)