Abstract | OBJECTIVE: DESIGN: Controlled in vivo laboratory study. SETTING: Animal research laboratory. SUBJECTS: One hundred seventy-seven male adult Sprague-Dawley rats weighing 300 to 370 g. INTERVENTIONS: The endovascular perforation model of subarachnoid hemorrhage was produced. Subarachnoid hemorrhage or sham-operated rats were treated with an equal volume (1 microL) of pre- subarachnoid hemorrhage intracerebroventricular administration of two dosages (0.02 and 0.1 microg) of recombinant osteopontin, albumin, or vehicle. Body weight, neurologic scores, brain edema, and blood-brain barrier disruption were evaluated, and Western blot analyses were performed to determine the effect of recombinant osteopontin on matrix metalloproteinase-9, substrates of matrix metalloproteinase-9 ( zona occludens-1, laminin), tissue inhibitor of matrix metalloproteinase-1, inflammation (interleukin-1beta), and nuclear factor-kappaB signaling pathways. MEASUREMENTS AND MAIN RESULTS: CONCLUSIONS:
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Authors | Hidenori Suzuki, Robert Ayer, Takashi Sugawara, Wanqiu Chen, Takumi Sozen, Yu Hasegawa, Kenji Kanamaru, John H Zhang |
Journal | Critical care medicine
(Crit Care Med)
Vol. 38
Issue 2
Pg. 612-8
(Feb 2010)
ISSN: 1530-0293 [Electronic] United States |
PMID | 19851092
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Interleukin-1
- NF-kappa B
- Neuroprotective Agents
- Recombinant Proteins
- Osteopontin
- Matrix Metalloproteinase 9
- Matrix Metalloproteinase 1
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Topics |
- Animals
- Blood-Brain Barrier
(metabolism)
- Blotting, Western
- Brain Injuries
(drug therapy, metabolism)
- Disease Models, Animal
- Injections, Intraventricular
- Interleukin-1
(biosynthesis)
- Male
- Matrix Metalloproteinase 1
(biosynthesis)
- Matrix Metalloproteinase 9
(biosynthesis)
- NF-kappa B
(biosynthesis)
- Neuroprotective Agents
(therapeutic use)
- Osteopontin
(therapeutic use)
- Rats
- Rats, Sprague-Dawley
- Recombinant Proteins
(therapeutic use)
- Subarachnoid Hemorrhage
(drug therapy, metabolism)
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