Nestin and SOX9 and SOX10 transcription factors are coexpressed in melanoma.

Abstract

Nestin is an intermediate filament expressed in proliferating neural progenitor cells and has been considered as a stem cell marker. Nestin is also found in melanoma and we recently demonstrated that its expression in melanoma cell lines is regulated by the transcription factors SOX9 and SOX10, but not BRN2. In this study, the expression levels of nestin, BRN2, SOX9 and SOX10 were analysed in tissues of melanoma (n = 78) and melanocytic nevi (n = 26) by immunohistochemistry. All proteins were highly expressed in primary and metastatic melanomas and, apart from BRN2, showed much lower levels in melanocytic nevi. Significant coexpression of nestin with SOX9 and SOX10 was found in primary melanoma confirming our in vitro data. Correlation analysis with clinicopathological data revealed that nestin was significantly associated with presence of ulceration in primary tumors and SOX9 with more advanced stage of disease. Our data reveal that SOX9 and SOX10 are highly expressed in melanoma and seem to have a regulatory role in nestin expression. The association with ulceration and advanced-stage tumors, respectively, suggests that nestin and SOX9 may be negative prognostic markers in melanoma.

• Authors: Renato M Bakos, Tanja Maier, Robert Besch, Dominik S Mestel, Thomas Ruzicka, Richard A Sturm, Carola Berking
• Journal: Experimental dermatology (Exp Dermatol) Vol. 19 Issue 8 Pg. e89-94 (Aug 2010) ISSN: 1600-0625 [Electronic] Denmark
• PMID: 19845757 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Homeodomain Proteins
• Intermediate Filament Proteins
• NES protein, human
• Nerve Tissue Proteins
• Nestin
• POU Domain Factors
• SOX10 protein, human
• SOX9 Transcription Factor
• SOX9 protein, human
• SOXE Transcription Factors
• transcription factor Brn-2

Topics

• Biomarkers, Tumor (metabolism)
• Biopsy
• Homeodomain Proteins (metabolism)
• Humans
• Intermediate Filament Proteins (metabolism)
• Melanoma (metabolism, pathology)
• Neoplasm Metastasis
• Nerve Tissue Proteins (metabolism)
• Nestin
• POU Domain Factors (metabolism)
• SOX9 Transcription Factor (metabolism)
• SOXE Transcription Factors (metabolism)
• Skin Neoplasms (metabolism, pathology)