Marine compound Xyloketal B protects PC12 cells against OGD-induced cell damage.

Abstract

Xyloketal B is a novel marine compound with unique chemical structure isolated from mangrove fungus Xylaria sp. (no. 2508). Recently, we have demonstrated that Xyloketal B is an antioxidant and can protect against oxidized low density lipoprotein (LDL)-induced cell injury. In the present study, we investigated whether Xyloketal B can protect against ischemia-induced cell injury in an in vitro oxygen glucose deprivation (OGD) model of ischemic stroke in PC12 cells. We found that Xyloketal B could directly scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and protect PC12 cells against OGD insult. Furthermore, Xyloketal B alleviated OGD-induced mitochondria superoxide, mitochondria fragmentation and GTPase dynamin-related protein 1 (Drp1) overexpression as well as reduction of mitochondrial membrane potential. All together, the present study demonstrates that Xyloketal B protects PC12 cells against OGD-induced cell injury and that the anti-oxidative property and protective action on mitochondria may account for its neuroprotective actions.

• Authors: Jia Zhao, Ling Li, Chen Ling, Jie Li, Ji-Yan Pang, Yong-Cheng Lin, Jie Liu, Ruxun Huang, Guan-Lei Wang, Zhong Pei, Jinsheng Zeng
• Journal: Brain research (Brain Res) Vol. 1302 Pg. 240-7 (Dec 11 2009) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 19765563 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Free Radical Scavengers
• Free Radicals
• Neuroprotective Agents
• Pyrans
• xyloketal B

Topics

• Animals
• Antioxidants (pharmacology, therapeutic use)
• Brain (drug effects, metabolism, physiopathology)
• Brain Ischemia (drug therapy, metabolism, physiopathology)
• Cell Death (drug effects, physiology)
• Free Radical Scavengers (pharmacology, therapeutic use)
• Free Radicals (antagonists & inhibitors, metabolism)
• Models, Biological
• Nerve Degeneration (drug therapy, metabolism, physiopathology)
• Neurons (drug effects, metabolism)
• Neuroprotective Agents (pharmacology, therapeutic use)
• Oxidative Stress (drug effects, physiology)
• PC12 Cells
• Pyrans (pharmacology, therapeutic use)
• Rats
• Stroke (drug therapy, metabolism, physiopathology)