Abstract | OBJECTIVE: METHODS: Thirty-six Wistar rats were equally randomized into control, model and EA groups. CIA model ( rheumatoid arthritis) was duplicated by intradermal injection of Bovine type II collagen into the back of the anesthetized rats. EA (2 Hz, 2 mA) was applied to "Zusanli" (ST 36) and "Sanyinjiao" ( SP 6) for 30 min, once a day for 30 days. The paw volume was measured by using a Plethysmometer and hot water tail-flick tests (50 degrees C) were conducted for detecting the rats' pain threshold (PT) before and after the treatment. The contents of PGE2, IL-1beta and TNF-alpha in supernatant of the cultured joint synoviocytes were detected by enzyme linked immunosorbent assay (ELISA). RESULTS: In comparison with control group, the swelled paw volume, the declined PT, PGE2, TNF-alpha and IL-1beta contents in knee-joint synoviocyte supernatant were significantly higher in model group (P < 0.05). Compared with model group, PT increased obviously (P < 0.05); paw volume, supernatant PGE2, TNF-alpha and IL-1beta contents decreased obviously (P < 0.05) in EA group. CONCLUSION: EA of ST 36 and SP 6 has a pronounced therapeutic effect in relieving knee joint pain and inflammation in CIA rats, which may be related to its effect in regulating the secretory function of the knee-joint synoviocytes.
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Authors | Jian-Qiao Fang, Xiao-Mei Shao, Gui-Zhi Ma |
Journal | Zhen ci yan jiu = Acupuncture research
(Zhen Ci Yan Jiu)
Vol. 34
Issue 2
Pg. 93-6
(Apr 2009)
ISSN: 1000-0607 [Print] China |
PMID | 19685721
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-1beta
- Tumor Necrosis Factor-alpha
- Dinoprostone
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Topics |
- Acupuncture Points
- Animals
- Arthritis, Experimental
(metabolism, therapy)
- Dinoprostone
(analysis)
- Electroacupuncture
- Interleukin-1beta
(analysis)
- Knee Joint
(cytology, metabolism)
- Male
- Pain Threshold
- Rats
- Rats, Wistar
- Synovial Fluid
(metabolism)
- Tumor Necrosis Factor-alpha
(analysis)
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