Abstract | AIMS: MAIN METHODS: KL-6 mucin expressions in 21 patients with CC, 12 with combined hepatocellular and cholangiocarcinoma (cHCC-CC), and 78 with hepatocellular carcinoma (HCC) were detected by immunohistochemical staining. The effects of two glycosylation inhibitors ( tunicamycin and benzyl-alpha-N-acetylgalactosamine (BAG)) on CC cell proliferations were assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyl-tetrazolium bromide (MTT) assays. KL-6 mucin expressions were detected by immunocytochemical staining and western blotting after tunicamycin or BAG treatment. Cell adhesive and invasive properties were evaluated by adhesion tests and transwell chamber assays after tunicamycin or BAG treatment. KEY FINDINGS: Positive KL-6 mucin staining was observed in all CC tissues and CC areas of cHCC-CC tissues. Immunocytochemical staining and western blotting showed that KL-6 mucin expressions were significantly reduced after both inhibitors treatment. Cell adhesive properties were significantly decreased after both inhibitors treatment, while cell invasive abilities were significantly decreased after BAG but not tunicamycin treatment. SIGNIFICANCE: This study indicated that KL-6 mucin might be a specific tumor target for CC. Therapeutic strategies that target glycosylation of KL-6 mucin may be useful to control aggressive behaviors of CC.
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Authors | H L Xu, Y Inagaki, Y Seyama, Y Sugawara, N Kokudo, M Nakata, F S Wang, W Tang |
Journal | Life sciences
(Life Sci)
Vol. 85
Issue 9-10
Pg. 395-400
(Aug 26 2009)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 19631667
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- MUC1 protein, human
- Mucin-1
- Tunicamycin
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Anti-Bacterial Agents
(pharmacology)
- Bile Ducts, Intrahepatic
(metabolism)
- Cell Adhesion
(drug effects, physiology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cholangiocarcinoma
(physiopathology)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Liver Neoplasms
(physiopathology)
- Male
- Middle Aged
- Mucin-1
(metabolism)
- Tunicamycin
(pharmacology)
- Young Adult
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