We describe the cases of two unrelated patients who exhibited multiple
chromosomal abnormalities in donor cells after allogeneic
peripheral blood stem cell transplantation (PBSCT). The patients were diagnosed with
chronic myeloid leukemia and
chronic lymphocytic leukemia, respectively, and both underwent nonmyeloablative conditioning with
cyclophosphamide and
fludarabine followed by PBSCT from their HLA-matched opposite-sex siblings. Post-transplant bone marrow cytogenetics showed full engraftment, and the early post-transplant studies demonstrated only normal donor metaphases. Subsequent studies of both patients, however, revealed a population of metaphase cells with abnormal, but apparently balanced, donor karyotypes. Chromosome studies performed on peripheral blood cells collected from both donors after
transplantation were normal. Both patients remained in clinical remission during follow-up of approximately 8 years in one case, and 6 years in the other case, despite the persistence of the abnormal clones.
Chromosomal abnormalities in residual recipient cells after bone marrow or PBSCT are not unusual. In contrast, only rare reports of
chromosome abnormalities in donor cells exist, all of which have been associated with post-bone marrow transplant
myelodysplastic syndrome or acute
leukemias. The present cases demonstrate the rare phenomenon of persistent clonal nonpathogenic
chromosome aberrations in cells of donor origin.