Differential cytokine expressions affect the severity of peri-implant disease.

Abstract

OBJECTIVE:

This study assessed gene expression by quantitative polymerase chain reaction of inflammatory- [interleukin (IL)-12, tumor necrosis factor-alpha (TNF-alpha), IL-4, and IL-10] and osteoclastogenesis-related factors [receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin (OPG)] in sites exhibiting different severities of peri-implant disease.

MATERIAL AND METHODS:

Peri-implant soft tissue biopsies (n=48) were harvested from healthy implant (HI), mucositis (MC), initial peri-implantitis (IP) and severe peri-implantitis (SP) sites.

RESULTS:

IL-12 and TNF-alpha mRNA levels were higher in SP, followed by IP and MC (P <0.05). IL-4 was higher in HI, followed by MC, SP and IP (P <0.05). IL-10 was the lowest in HI, while no differences were detected among the diseased groups (P>0.05). OPG mRNA levels were higher in HI, followed by IP, SP and MC, whereas RANKL was increased as the peri-implantitis severity increased (P<0.05). The highest OPG/RANKL ratio was observed in HI and the lowest in SP (P<0.01).

CONCLUSION:

These findings suggest that expressions of inflammatory- and osteoclastogenesis-related factors may play an important role in the onset and severity of the peri-implant diseases.

• Authors: Poliana Mendes Duarte, Adriana Cutrim de Mendonça, Maria Beatriz Braz Máximo, Vanessa Renata Santos, Marta Ferreira Bastos, Francisco Humberto Nociti Júnior
• Journal: Clinical oral implants research (Clin Oral Implants Res) Vol. 20 Issue 5 Pg. 514-20 (May 2009) ISSN: 1600-0501 [Electronic] Denmark
• PMID: 19302394 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Dental Implants
• Osteoprotegerin
• RANK Ligand
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Interleukin-12
• Interleukin-4

Topics

• Adult
• Analysis of Variance
• Cell Differentiation (immunology)
• Cytokines (immunology, metabolism)
• Dental Implants (adverse effects)
• Female
• Gene Expression Regulation (immunology, physiology)
• Humans
• Interleukin-10 (genetics, metabolism)
• Interleukin-12 (genetics, metabolism)
• Interleukin-4 (genetics, metabolism)
• Jaw, Edentulous (immunology, rehabilitation)
• Male
• Middle Aged
• Mucositis (etiology, immunology, metabolism)
• Osteoclasts (immunology, metabolism)
• Osteoprotegerin (genetics, metabolism)
• Periodontitis (etiology, immunology, metabolism)
• RANK Ligand (genetics, metabolism)
• RNA, Messenger (analysis)
• Reference Values
• Severity of Illness Index
• Tumor Necrosis Factor-alpha (genetics, metabolism)