Albumin ameliorates tissue plasminogen activator-mediated blood-brain barrier permeability and ischemic brain injury in rats.

Abstract

OBJECTIVE:

Tissue plasminogen activator (tPA) may aggravate ischemic neuronal damage after focal cerebral ischemia and increase blood-brain barrier permeability. Human serum albumin has neuroprotective effects on ischemic stroke. However, whether albumin can attenuate the deleterious effects of tPA is yet unknown.

METHODS:

In the present study, we attempted to determine the effects of albumin on cerebral injury and blood-brain barrier disruption induced by middle cerebral artery occlusion for 2 hours followed by 24 hours of reperfusion and tPA.

RESULTS:

We found that infarct volume in rats which received saline and tPA was 35.6 +/- 3.8% (mean +/- SEM) and 50.9 +/- 4.5%, respectively. There was significant difference between the two groups (p<0.01). The infarct volume in rats that received tPA with albumin was significantly decreased to 29.2 +/- 3.3% (p<0.05), compared with tPA-only-treated group. Combination therapy using tPA with albumin also improved neurological deficits and reduced brain edema significantly (p<0.05). Relative to tPA-treated group, rats that received combination therapy using tPA with albumin had significantly reduced blood-brain barrier permeability, evaluated by quantitation of Evans blue leakage (p<0.05).

CONCLUSION:

In acute ischemic stroke, combination therapy using tPA with albumin can attenuate the deleterious effects of tPA.

• Authors: Jin Tang, Ya-Jun Li, Jun Mu, Qi Li, De-Yu Yang, Peng Xie
• Journal: Neurological research (Neurol Res) Vol. 31 Issue 2 Pg. 189-94 (Mar 2009) ISSN: 0161-6412 [Print] England
• PMID: 19298760 (Publication Type: Journal Article)

Chemical References

• Albumins
• Fibrinolytic Agents
• Tetrazolium Salts
• triphenyltetrazolium
• Tissue Plasminogen Activator

Topics

• Albumins (therapeutic use)
• Analysis of Variance
• Animals
• Blood-Brain Barrier (drug effects, physiopathology)
• Brain Edema (drug therapy, etiology, pathology)
• Brain Injuries (drug therapy, etiology, pathology)
• Capillary Permeability (drug effects)
• Cell Count (methods)
• Cell Death (drug effects)
• Disease Models, Animal
• Fibrinolytic Agents (adverse effects, pharmacology)
• In Situ Nick-End Labeling (instrumentation)
• Infarction, Middle Cerebral Artery (complications)
• Male
• Neurologic Examination
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (chemically induced, drug therapy)
• Tetrazolium Salts
• Tissue Plasminogen Activator (adverse effects, pharmacology)