Photoprotective effect of Pothomorphe umbellata on UVB radiation-induced biomarkers involved in carcinogenesis of hairless mouse epidermis.

Abstract

In this study we assessed the protective effect of topical application of Pothomorphe umbellata extract on ultraviolet B (UVB)-induced skin lesion parameters in hairless mouse epidermis. A single dose of UVB irradiation (0.23 kJ/m2) resulted in a significant decrease in thymine dimer-positive cells and apoptotic sunburn cells, with an increase in p53 and proliferating cell nuclear antigen-positive cells in the epidermis. After 5 weeks (total dose 13.17 kJ/m2) and 15 weeks (total dose 55.51 kJ/m2) of irradiation, P. umbellata treatment inhibited the hyperplasic response and induced an increase in p53-positive cells. These findings suggest that P. umbellata extract affords protection against UVB-induced skin lesions.

• Authors: Vanessa Vitoriano da Silva, Cristina Dislich Ropke, Denise Varella Miranda, Rebeca Leite de Almeida, Tânia Cristina Higashi Sawada, Diogo Pineda Rivelli, Silvia Berlanga de Moraes Barros
• Journal: Cutaneous and ocular toxicology (Cutan Ocul Toxicol) Vol. 28 Issue 2 Pg. 54-60 ( 2009) ISSN: 1556-9535 [Electronic] England
• PMID: 19263261 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Plant Extracts
• Proliferating Cell Nuclear Antigen
• Pyrimidine Dimers
• Radiation-Protective Agents
• Tumor Suppressor Protein p53

Topics

• Administration, Cutaneous
• Animals
• Apoptosis (drug effects, radiation effects)
• Biomarkers (metabolism)
• Carcinogenicity Tests
• Epidermis (drug effects, metabolism, pathology, radiation effects)
• Female
• Hyperplasia
• Immunohistochemistry
• Mice
• Mice, Hairless
• Neoplasms, Radiation-Induced (etiology, metabolism, pathology, prevention & control)
• Piperaceae (chemistry)
• Plant Extracts (administration & dosage, isolation & purification, therapeutic use)
• Proliferating Cell Nuclear Antigen (metabolism)
• Pyrimidine Dimers (metabolism)
• Radiation-Protective Agents (administration & dosage, isolation & purification, therapeutic use)
• Skin Neoplasms (etiology, metabolism, pathology, prevention & control)
• Tumor Suppressor Protein p53 (biosynthesis)
• Ultraviolet Rays (adverse effects)