Passive transfer of Plasmodium falciparum MSP-2 pseudopeptide-induced antibodies efficiently controlled parasitemia in Plasmodium berghei-infected mice.

Abstract

We have developed monoclonal antibodies directed against the pseudopeptide psi-130, derived from the highly conserved malarial antigen Plasmodium falciparum merozoite surface protein 2 (MSP-2), for obtaining novel molecular tools with potential applications in the control of malaria. Following isotype switching, these antibodies were tested for their ability to suppress blood-stage parasitemia through passive immunization in malaria-infected mice. Some proved totally effective in suppressing a lethal blood-stage challenge infection and others reduced malarial parasitemia. Protection against P. berghei malaria following Ig passive immunization can be associated with specific immunoglobulins induced by a site-directed designed MSP-2 reduced amide pseudopeptide.

• Authors: Paola A Martínez, Nubia Yandar, Liliana P Lesmes, Martha Forero, Oscar Pérez-Leal, Manuel Elkin Patarroyo, José Manuel Lozano
• Journal: Peptides (Peptides) Vol. 30 Issue 2 Pg. 330-42 (Feb 2009) ISSN: 0196-9781 [Print] United States
• PMID: 19071172 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Protozoan
• Antigens, Protozoan
• Protozoan Proteins
• Recombinant Proteins
• merozoite surface protein 2, Plasmodium

Topics

• Animals
• Antibodies, Protozoan (immunology, therapeutic use)
• Antigens, Protozoan (chemistry, genetics, immunology)
• Disease Models, Animal
• Female
• Immunization, Passive
• Malaria (drug therapy)
• Mice
• Mice, Inbred BALB C
• Parasitemia (drug therapy)
• Plasmodium berghei
• Plasmodium falciparum (immunology)
• Protozoan Proteins (chemistry, genetics, immunology)
• Recombinant Proteins (genetics, metabolism)