Abstract | BACKGROUND: METHODS: Hartley male guinea pigs, weighing between 250 g and 350 g, were injected with LPS at a dose of 1 mg/kg every 24 hours for three days. A non-selective NOS inhibitor, N(omega)-nitro-L-arginine methyl ester ( L-NAME), or a selective inducible NOS inhibitor, aminoguanidine (AG), were used thirty minutes before each injection of LPS. Airway reactions, nitric oxide (NO) production and inflammatory changes were detected 24 hours after the last dose of LPS. RESULTS: AG significantly decreased the NO production in the bronchoalveolar lavage fluid (BALF) and sharply reduced the intensity of bronchoconstriction to histamine challenge. L-NAME also significantly decreased the NO production in the BALF, but had no effect on airway reactions or, perhaps, a tendency to enhance the intensity of AHR. CONCLUSIONS: The data suggest that inducible NOS contributes to the AHR induced by repetitive intraperitoneal LPS, and constitutive NOS was also involved.
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Authors | Hong-Ni Jiang, Jie-Ming Qu, Li-Xian He, Xue-Hua Chen, Jue Pan, Li Li, Da-Nian Zhu, Yin-Xiang Cao, Lin-Lin Shen |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 121
Issue 17
Pg. 1693-7
(Sep 05 2008)
ISSN: 0366-6999 [Print] China |
PMID | 19024101
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Enzyme Inhibitors
- Guanidines
- Lipopolysaccharides
- Nitric Oxide
- Nitric Oxide Synthase
- pimagedine
- NG-Nitroarginine Methyl Ester
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Topics |
- Airway Resistance
(drug effects)
- Animals
- Bronchial Hyperreactivity
(chemically induced)
- Enzyme Inhibitors
(pharmacology)
- Guanidines
(pharmacology)
- Guinea Pigs
- Lipopolysaccharides
(toxicity)
- Male
- NG-Nitroarginine Methyl Ester
(pharmacology)
- Nitric Oxide
(biosynthesis)
- Nitric Oxide Synthase
(antagonists & inhibitors, physiology)
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