A new rat colon cancer cell line metastasizes spontaneously: biologic characteristics and chemotherapeutic response.

Abstract

A new cell line (RCN-9) was established in culture from a transplantable rat colon adenocarcinoma, which was induced in the colon of a male Fischer F344 rat by subcutaneous administration of 1,2-dimethylhydrazine. When RCN-9 cells were injected subcutaneously or into the cecal subserosa of syngeneic rats, carcinomas with progressive growth were obtained and the development of lung (63.6%) and liver (40.0%) metastases, respectively, ensued. Antitumor effects of 5-fluorouracil (5-FU), adriamycin (ADM) and mitomycin C (MMC) against RCN-9 were examined in vivo and in vitro. 5-FU and ADM had antitumor effects both in vivo and in vitro; MMC had antitumor effects in vitro. These results show that the RCN-9 cell line can be used both as a model to study mechanisms of metastasis from colon carcinoma and as a model in chemotherapeutic studies of metastatic disease from colon carcinoma.

• Authors: Y Inoue, Y Kashima, K Aizawa, K Hatakeyama
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 82 Issue 1 Pg. 90-7 (Jan 1991) ISSN: 0910-5050 [Print] Japan
• PMID: 1900274 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Adenocarcinoma (drug therapy, pathology)
• Animals
• Colonic Neoplasms (drug therapy, pathology)
• Graft Survival
• Liver Neoplasms (secondary)
• Lung Neoplasms (secondary)
• Male
• Neoplasm Transplantation
• Rats
• Rats, Inbred F344
• Tumor Cells, Cultured (pathology)