Cell cycle-dependent variation of a CD133 epitope in human embryonic stem cell, colon cancer, and melanoma cell lines.

Abstract	CD133 (Prominin1) is a pentaspan transmembrane glycoprotein expressed in several stem cell populations and cancers. Reactivity with an antibody (AC133) to a glycoslyated form of CD133 has been widely used for the enrichment of cells with tumor-initiating activity in xenograph transplantation assays. We have found by fluorescence-activated cell sorting that increased AC133 reactivity in human embryonic stem cells, colon cancer, and melanoma cells is correlated with increased DNA content and, reciprocally, that the least reactive cells are in the G(1)-G(0) portion of the cell cycle. Continued cultivation of cells sorted on the basis of high and low AC133 reactivity results in a normalization of the cell reactivity profiles, indicating that cells with low AC133 reactivity can generate highly reactive cells as they resume proliferation. The association of AC133 with actively cycling cells may contribute to the basis for enrichment for tumor-initiating activity.
Authors	Marie Jaksch, Jorge Múnera, Ruchi Bajpai, Alexey Terskikh, Robert G Oshima
Journal	Cancer research (Cancer Res) Vol. 68 Issue 19 Pg. 7882-6 (Oct 01 2008) ISSN: 1538-7445 [Electronic] United States
PMID	18829544 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References	AC133 Antigen Antigens, CD Epitopes Glycoproteins PROM1 protein, human Peptides Prom1 protein, mouse DNA
Topics	AC133 Antigen Animals Antigens, CD (immunology, metabolism) Caco-2 Cells Cell Cycle (immunology, physiology) Cell Line, Tumor Cell Proliferation Cell Separation (methods) Coculture Techniques Colonic Neoplasms (metabolism, pathology) DNA (analysis) Embryonic Stem Cells (metabolism) Epitopes (metabolism) Gene Expression Profiling Glycoproteins (immunology, metabolism) Humans Immunohistochemistry Melanoma (metabolism, pathology) Mice Peptides (immunology, metabolism)

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