Brain death affects the hepatic immunogenicity of pigs.

Abstract

OBJECTIVE:

The purpose of this study was to observe how the brain-death state affects the hepatic immunogenicity of pigs.

MATERIALS AND METHODS:

Twelve healthy ChangBai pigs were allocated randomly to 2 groups: control group (n = 6) with Foley catheter balloon placed intracalvarium for 24 hours only versus brain-death group (n = 6) with brain death for 24 hours. The serum and hepatic tissues were obtained at 6, 12, and 24 hours after the initial confirmation of brain death. Tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were determined by enzyme-linked immunosorbent assay (ELISA). The major histocompatibility complex class II (MHC-II) was determined by immunohistochemistry, and the intercellular adhesion molecule-1 (ICAM-1) mRNA and monocyte chemotactic protein-1 (MCP-1) mRNA by real-time polymerase chain reaction (PCR).

RESULTS:

The serum TNF-alpha and IL-6, MHC-II, ICAM-1 mRNA, and MCP-1 mRNA in hepatic tissues in the brain-death group were higher than in the control group; they all increased with time.

CONCLUSION:

Brain death increased liver immunogenicity, which may increase the immune lesion and rejection after transplantation.

• Authors: S Zhang, C Zhu, Y Wu, W Zhai, J Shi, Y Song, J Li, Z Li, G Zhang
• Journal: Transplantation proceedings (Transplant Proc) Vol. 40 Issue 7 Pg. 2159-62 (Sep 2008) ISSN: 0041-1345 [Print] United States
• PMID: 18790180 (Publication Type: Journal Article)

Chemical References

• Histocompatibility Antigens Class II
• Interleukin-6
• Receptors, CCR2
• Tumor Necrosis Factor-alpha
• Intercellular Adhesion Molecule-1

Topics

• Animals
• Brain Death (metabolism, pathology)
• Histocompatibility Antigens Class II (metabolism)
• Intercellular Adhesion Molecule-1 (genetics)
• Interleukin-6 (blood)
• Kinetics
• Liver (immunology)
• Receptors, CCR2 (genetics)
• Swine
• Tumor Necrosis Factor-alpha (metabolism)