[Effect of petroleum ether extract from Melilotus suaveolens Ledeb on the expression of NF-kappaB and Heme oxygenase 1].

Abstract

AIM:

To study the anti-inflammatory effect of petroleum ether extract from Melilotus suaveolens Ledeb.

METHODS:

Inflammatory cell model was constructed by LPS acting on the RAW264.7 cell line. The expression and distribution of NF-kappaB were detected using immunocytochemical method. The expression of mRNA and protein of Heme oxygenase 1(HO-1) were detected by real-time PCR and Western blot.

RESULTS:

The immunocytochemical analysis showed that the cytoplasm stained to brown presented NF-kappaB inactivation after the intervention of petroleum ether extract while the cell nucleus stained to brown presented NF-kappaB activation after the only intervention of LPS. The expression of HO-1 mRNA was significantly enhanced by the extract in a dose-dependent manner, and the expression of HO-1 protein was markedly enhanced too.

CONCLUSION:

The petroleum ether extract from Melilotus suaveolens Ledeb can resist inflammation by inhibiting the activation of proinflammatory factor NF-kappaB and enhancing the expression of anti-inflammatory factor HO-1.

• Authors: Ran Pang, Shu-ling Zhang, Lei Zhao, Shuang-lin Liu, Ji-hua Dong, Jun-yan Tao
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 24 Issue 9 Pg. 861-3 (Sep 2008) ISSN: 1007-8738 [Print] China
• PMID: 18782516 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• NF-kappa B
• Plant Extracts
• Ether
• Heme Oxygenase-1

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cell Line, Tumor
• Ether (chemistry)
• Gene Expression (drug effects)
• Heme Oxygenase-1 (genetics, immunology)
• Inflammation (drug therapy, genetics, immunology)
• Melilotus (chemistry)
• Mice
• NF-kappa B (genetics, immunology)
• Plant Extracts (pharmacology)