In vitro differentiation of human monocytes into dendritic cells by peptic-tryptic digest of gliadin is independent of genetic predisposition and the presence of celiac disease.

Abstract

INTRODUCTION:

This study was done to further reveal the role of the innate immune system in celiac disease.

METHODS:

Dendritic cells were matured from venous blood of patients with active or treated celiac disease and DQ2-DQ8-positive or negative controls. Dendritic cells were treated with a peptic-tryptic digest of gliadin (500 microg/ml) and their activation was analyzed by fluorescent-activated cell sorting analysis, cytokine secretion, and their ability to elicit T cell proliferation.

RESULTS AND DISCUSSION:

Gliadin upregulated interleukin (IL)-6, IL-8, and IL-12 (p40) secretion in dendritic cells and induced strong expression of the maturation markers human leukocyte antigen (HLA)-DR, CD25, CD83, and CD86 of all subjects irrespective of their genotype or the presence of disease, whereas the digest of bovine serum albumin showed no effect. However, gliadin-stimulated dendritic cells from active celiac showed enhanced stimulation of autologous T cells compared to the other groups.

CONCLUSION:

Further research should be aimed at identifying the mechanisms that control inflammation in healthy individuals.

• Authors: Maryam Rakhimova, Birgit Esslinger, Anja Schulze-Krebs, Eckhart G Hahn, Detlef Schuppan, Walburga Dieterich
• Journal: Journal of clinical immunology (J Clin Immunol) Vol. 29 Issue 1 Pg. 29-37 (Jan 2009) ISSN: 1573-2592 [Electronic] Netherlands
• PMID: 18696220 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• B7-2 Antigen
• Cytokines
• HLA-DR Antigens
• Immunoglobulins
• Interleukin-2 Receptor alpha Subunit
• Membrane Glycoproteins
• Gliadin
• Trypsin

Topics

• Adult
• Aged
• Antigens, CD (immunology, metabolism)
• B7-2 Antigen (immunology, metabolism)
• Celiac Disease (genetics, immunology, metabolism)
• Cytokines (biosynthesis, immunology)
• Dendritic Cells (drug effects, immunology, metabolism)
• Female
• Genetic Predisposition to Disease
• Genotype
• Gliadin (chemistry, immunology, pharmacology)
• HLA-DR Antigens (immunology, metabolism)
• Humans
• Immunoglobulins (immunology, metabolism)
• Interleukin-2 Receptor alpha Subunit (immunology, metabolism)
• Male
• Membrane Glycoproteins (immunology, metabolism)
• Middle Aged
• Monocytes (drug effects, immunology, metabolism)
• T-Lymphocytes (immunology, metabolism)
• Trypsin (chemistry)
• Up-Regulation (immunology)
• CD83 Antigen