Abstract |
Japanese encephalitis is a severe central nervous system ( CNS) disease with a high case fatality rate in humans. We characterized T-cells infiltrating the brain after infection with Japanese encephalitis virus (JEV) in a mouse model and determined the clonality of the infiltrating T-cells by analyzing the sequences of complementary determining region 3 (CDR3) of the T-cell receptor. C3H/He mice died after intraperitoneal infection with the JaTH160 strain of JEV, demonstrating CNS degeneration and prominent T-cell infiltration. The percentages of T-cells bearing the VA5-1, VA17-1, VA19-1, VB2-1, VB8-3 and VB13-1 subfamilies were significantly increased following infiltration of the brains in infected mice. Additionally, CDR3 size spectratyping revealed the oligoclonality in T-cells bearing VA11-1 and VA18-1. CDR3 amino acid sequences were then determined for the VA5-1, VA11-1, VA18-1, VB8-3 and VB13-1 subfamilies. There were high levels of identity and similarity in amino acid sequences of CDR3 in these T-cells. Quantitative real-time PCR analysis also revealed that CD8, interferon-gamma and tumor necrosis factor-alpha were highly expressed in the infected mouse brain. These results indicate that T-cells with high clonality and similarity infiltrate the JEV-infected mouse brain, and that these T-cells are mainly CD8-positive and have the Th1/Tc1 phenotype.
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Authors | Yoshiki Fujii, Kazutaka Kitaura, Kazuo Nakamichi, Tomohiko Takasaki, Ryuji Suzuki, Ichiro Kurane |
Journal | Japanese journal of infectious diseases
(Jpn J Infect Dis)
Vol. 61
Issue 1
Pg. 40-8
(Jan 2008)
ISSN: 1344-6304 [Print] Japan |
PMID | 18219133
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Complementarity Determining Regions
- Cytokines
- Receptors, Antigen, T-Cell
- Tumor Necrosis Factor-alpha
- Interferon-gamma
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Topics |
- Amino Acid Sequence
- Animals
- Brain
(immunology, pathology, virology)
- CD8-Positive T-Lymphocytes
(immunology)
- Complementarity Determining Regions
(chemistry)
- Cytokines
(blood, metabolism)
- Encephalitis Virus, Japanese
(immunology)
- Encephalitis, Japanese
(immunology, mortality, pathology)
- Female
- Interferon-gamma
(metabolism)
- Mice
- Mice, Inbred C3H
- Receptors, Antigen, T-Cell
(analysis)
- T-Lymphocytes
(immunology, virology)
- Tumor Necrosis Factor-alpha
(metabolism)
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