Multipoint quantification of multimarker genes in peripheral blood and micrometastasis characteristic in peri-operative esophageal cancer patients.
|
| Abstract |
This work proposed a method to assess the occult micrometastasis characteristic in peri-operative peripheral blood (PB) based on multipoint quantification of multimarker genes by real-time semiquantitative RT-PCR. The expression levels of SCC, CK19, CK20, CEA and survivin mRNA in PB samples collected before surgery (B-1), immediately after surgery (B0) and at the third day post-operatively (B+3) from 36 squamous esophageal cancer (EC) patients were detected. SCC and CK19 mRNA showed low positivity detection rates, while the rate for CEA and survivin mRNA panel was 58.3%, 83.3% and 72.3% at B-1, B0 and B+3, respectively. Opposite to the significant increase and slow decrease of CEA cells at stages of B-1 to B0 and B0 to B+3, respectively, survivin cells decreased significantly and increased quickly at the two stages. The follow-up with a period of 1.2 years showed that the patients with the B+3/B0 ratios of >or= 0.3 for CEA cells or/and >or= 10 for survivin cells exhibited significantly high possibility of developed metastasis, and the sensitivity to predict developed metastasis increased from 54.5% of CEA mRNA alone to 72.7% of CEA and survivin mRNA panel. These results suggested that CEA and survivin gene panel improved the sensitivity to predict recurrence and differentiate the micrometastatic process.
|
|---|---|
| Authors | Zhian Liu, Ming Jiang, Feng Yan, Lin Xu, Jianhua Zhao, Huangxian Ju |
| Journal | Cancer letters (Cancer Lett) Vol. 261 Issue 1 Pg. 46-54 (Mar 08 2008) ISSN: 0304-3835 [Print] Ireland |
| PMID | 18082938 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!