Induction of lytic cycle replication of Kaposi's sarcoma-associated herpesvirus by herpes simplex virus type 1: involvement of IL-10 and IL-4.

Abstract

Previously, we identified that both human herpesvirus 6 and human immunodeficiency virus type 1 Tat were important cofactors that activated lytic cycle replication of Kaposi's sarcoma-associated herpesvirus (KSHV). Here, we further investigated the potential of herpes simplex virus type 1 (HSV-1) to influence KSHV replication. We demonstrated that HSV-1 was a potentially important factor in the pathogenesis of Kaposi's sarcoma, as determined by production of lytic phase mRNA transcripts, viral proteins and infectious viral particles in BCBL-1 cells. These results were further confirmed by an RNA interference experiment using small interfering RNA targeting KSHV ORF50 and a luciferase reporter assay testing ORF50 promoter-driven luciferase activity. Finally, we discovered that production of human interleukin-10 (IL-10) and IL-4 partially contributed to HSV-1-induced KSHV replication. Our data present the first direct evidence that HSV-1 can activate KSHV lytic replication and suggest a role of HSV-1 in KSHV pathogenesis.

• Authors: Di Qin, Yi Zeng, Chao Qian, Zan Huang, Zhigang Lv, Lin Cheng, Shuihong Yao, Qiao Tang, Xiuying Chen, Chun Lu
• Journal: Cellular microbiology (Cell Microbiol) Vol. 10 Issue 3 Pg. 713-28 (Mar 2008) ISSN: 1462-5822 [Electronic] India
• PMID: 18042256 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Viral
• Viral Proteins
• Interleukin-10
• Interleukin-4

Topics

• Animals
• Cell Line
• Gene Silencing
• Herpesvirus 1, Human (physiology)
• Herpesvirus 8, Human (physiology)
• Humans
• Interleukin-10 (metabolism)
• Interleukin-4 (metabolism)
• RNA, Viral (biosynthesis)
• Viral Proteins (biosynthesis)
• Virus Replication (physiology)