Preferential induction of transforming growth factor-beta production in gastric epithelial cells and monocytes by Helicobacter pylori soluble proteins.

Abstract

BACKGROUND:

The cytokines induced by Helicobacter pylori, as well as the intricate balance of proinflammatory and anti-inflammatory cytokines, are relevant to the outcomes of H. pylori infection. Transforming growth factor (TGF)-beta and interleukin (IL)-10 are 2 vital anti-inflammatory cytokines that regulate mucosal immunity in various inflammatory and infectious diseases.

METHODS:

To elucidate whether host-bacteria interaction can influence TGF-beta and IL-10 production, we investigated the expression of TGF-beta and IL-10 in various mammalian cell lines preincubated with H. pylori and other enteric bacteria.

RESULTS:

The amount of TGF-beta protein, but not IL-10, was significantly increased after stimulation with H. pylori, but other enteric bacteria did not induce TGF- beta production. Different H. pylori strains isolated from patients with gastritis, peptic ulcer, gastric cancer and strains with cagA or vacA isogenic mutations showed similar effects on TGF-beta induction, indicating that this effect was a constitutional characteristic of H. pylori and independent of cagA and vacA status.

CONCLUSION:

The results imply the presence of a protein factor (termed "TGF-beta-inducing protein") that induces production of TGF-beta. In view of the multiple effects of TGF-beta , we conclude the TGF-beta-inducing protein of H. pylori might mediate the immune response and contribute to the pathogenesis of H. pylori infection.

• Authors: Ming-Shiang Wu, Jaw-Town Lin, Ping-Ning Hsu, Ching-Yi Lin, Yuan-Ting Hsieh, Yi-Han Chiu, Po-Ren Hsueh, Kuang-Wen Liao
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 196 Issue 9 Pg. 1386-93 (Nov 01 2007) ISSN: 0022-1899 [Print] United States
• PMID: 17922404 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Bacterial Proteins
• Transforming Growth Factor beta
• VacA protein, Helicobacter pylori
• cagA protein, Helicobacter pylori
• Interleukin-10

Topics

• Antigens, Bacterial (genetics, metabolism)
• Bacterial Proteins (chemistry, genetics, metabolism)
• Cell Line, Tumor
• Epithelial Cells (metabolism)
• Helicobacter pylori (metabolism)
• Humans
• Interleukin-10 (metabolism)
• Monocytes (metabolism)
• Stomach (cytology)
• Transforming Growth Factor beta (biosynthesis)