Changes in the expression of
arginase and their association with nitrosative stress were investigated using an asthmatic model previously established in NC/Nga mice with mite extract. Mite
crude extract (100 microg/day) from Dermatophagoides farinae was administered intranasally for 5 consecutive days (day 0-4), and a single challenge was performed on day 11. On day 12, upregulation of the
mRNA expression of inducible types of
nitric oxide synthase (iNOS) and increases in immunohistochemical staining for iNOS and
nitrotyrosine were observed. However, the level of
nitrite +
nitrate was unchanged. An increase in enzymatic activity, upregulation of
mRNA expression, and immunostaining for
arginase I was detected in the lung tissue and serum. Moreover, increases in both
arginase I and II were revealed by immunoblotting. Goblet cell
hyperplasia in bronchial epithelial cells and increasing
collagen synthesis around the bronchus were also observed. These results suggested that an increase in
arginase may lead to decreased availability of
arginine for
nitric oxide synthase and may contribute to the remodeling of the lung.