Abstract |
Arthritis can be induced in mice by the injection of anti- type II collagen (anti-CII) Ab and LPS. To elucidate the role of IL-1 receptor antagonist (IL-1ra) in Ab-induced arthritis, WT and IL-1ra(-/-) mice were administered anti-CII Ab and LPS. These IL-1ra(-/-) mice developed severe arthritis even at low doses of anti-CII Ab and LPS, while WT mice did not. The cells that invaded the arthritic joints were mainly Gr-1(+) neutrophils, and the number of the cells in the joints remained high over 4 weeks in the IL-1ra(-/-) mice. KC, a ligand for CXCR2, is found in higher levels in the arthritic paws of IL-1ra(-/-) mice compared with the WT, and most of the cells that infiltrated into the joints of the IL-1ra(-/-) mice were CXCR2-expressing neutrophils. Administration of anti-CXCR2 Ab completely inhibited arthritis development. The anti-CXCR2 Ab decreased the number of neutrophils in the blood and also inhibited the migration of neutrophils to KC. These results suggested that the high susceptibility of IL-1ra(-/-) mice to anti-CII Ab-induced arthritis was due to the higher expression of chemotactic factors like KC and the sustained infiltration of CXCR2-expressing neutrophils into the joints.
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Authors | Takashi Kagari, Daisuke Tanaka, Hiromi Doi, Yoichiro Iwakura, Takaichi Shimozato |
Journal | European journal of immunology
(Eur J Immunol)
Vol. 37
Issue 10
Pg. 2753-63
(Oct 2007)
ISSN: 0014-2980 [Print] Germany |
PMID | 17823985
(Publication Type: Journal Article)
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Chemical References |
- Autoantibodies
- Collagen Type II
- Il1rn protein, mouse
- Interleukin 1 Receptor Antagonist Protein
- Lipopolysaccharides
- Receptors, Interleukin-8B
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Topics |
- Animals
- Arthritis, Experimental
(immunology, pathology)
- Arthritis, Rheumatoid
(immunology, pathology)
- Autoantibodies
(administration & dosage)
- Collagen Type II
(immunology)
- Interleukin 1 Receptor Antagonist Protein
(deficiency, genetics)
- Lipopolysaccharides
(immunology)
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Knockout
- Neutrophils
(immunology, metabolism)
- Receptors, Interleukin-8B
(biosynthesis, genetics)
- Severity of Illness Index
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