Alveolar macrophages are necessary for the systemic inflammation of acute alveolar hypoxia.

Abstract

Alveolar hypoxia (Fi(O(2)) 0.10) rapidly produces inflammation in the microcirculation of skeletal muscle, brain, and mesentery of rats. Dissociation between tissue Po(2) values and inflammation, plus the observation that plasma from hypoxic rats activates mast cells and elicits inflammation in normoxic tissues, suggest that the response to hypoxia is initiated when mast cells are activated by an agent released from a distant site and carried by the circulation. These experiments tested the hypothesis that this agent originates in alveolar macrophages (AM). Male rats were depleted of AM by tracheal instillation of clodronate-containing liposomes. Four days after treatment, AM recovered by bronchoalveolar lavage were <10% of control. Control rats received buffer-containing liposomes. As expected, alveolar hypoxia (Fi(O(2)) 0.10) in control rats increased leukocyte-endothelial adherence, produced degranulation of perivascular mast cells, and increased fluorescent albumin extravasation in the cremaster microcirculation. None of these effects was seen when AM-depleted rats were exposed to hypoxia. Plasma obtained from control rats after 5 min of breathing 10% O(2) elicited inflammation when applied to normoxic cremasters. In contrast, normoxic cremasters did not develop inflammation after application of plasma from hypoxic AM-depleted rats. Supernatant from AM cultured in 10% O(2) produced increased leukocyte-endothelial adherence, vasoconstriction, and albumin extravasation when applied to normoxic cremasters. Normoxic AM supernatant did not produce any of these responses. The effects of hypoxic supernatant were attenuated by pretreatment of the cremaster with the mast cell stabilizer cromolyn. These data support the hypothesis that AM are the source of the agent that initiates hypoxia-induced systemic inflammation by activating mast cells.

• Authors: Norberto C Gonzalez, Julie Allen, V Gustavo Blanco, Eric J Schmidt, Nico van Rooijen, John G Wood
• Journal: Journal of applied physiology (Bethesda, Md. : 1985) (J Appl Physiol (1985)) Vol. 103 Issue 4 Pg. 1386-94 (Oct 2007) ISSN: 8750-7587 [Print] United States
• PMID: 17656628 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Clodronic Acid

Topics

• Acute Disease
• Animals
• Brain (blood supply)
• Cell Adhesion (physiology)
• Cells, Cultured
• Clodronic Acid (pharmacology)
• Disease Models, Animal
• Endothelium, Vascular (pathology, physiology)
• Hypoxia (complications, metabolism, pathology)
• Leukocytes (pathology, physiology)
• Lung Diseases, Interstitial (pathology)
• Macrophages, Alveolar (drug effects, pathology, physiology)
• Male
• Mast Cells (pathology)
• Mesentery (blood supply)
• Microcirculation (drug effects, pathology)
• Muscle, Skeletal (blood supply)
• Pulmonary Alveoli (drug effects, pathology)
• Rats
• Rats, Sprague-Dawley
• Vasculitis (etiology, metabolism, pathology)