[Experimental model of liver oxidative damage induction in rats by halothane].

Abstract

BACKGROUND:

The anesthetic halothane can be reductively metabolized to reactives intermediates that may initiate lipid peroxidation accompanied by hepatic injury. Hypoxia and phenobarbital pretreatment in rats increases metabolism of halothane, the oxidative stress, cause liver antioxidant enzymes changes and tissue damage.

AIMS:

We investigated the effect of halothane on hepatic lipid peroxidation and on hepatic histology after increases reductive metabolism of halothane caused by hypoxia and phenobarbital pretreatment.

METHODS:

Twenty-five male wistar rats were divided in five equals groups: CO (Control), HO14 (Halothane/Hypoxia), F (fenobarbital alone), O14 (Hypoxia alone) and H (Halothane alone). After 24 hours the rats were killed, their livers removed to determine chemoluminescence, thiobarbituric acid-reactive substances, catalase, superoxide dismutase, and blood samples were taken to determine AST and ALT. The histopathologic evaluation was performed with hematoxylin and eosin staining. Histopathologic scores are presented as 25th-75th percentile/range values and median +/- range.

RESULTS/CONCLUSION:

Halothane-hypoxic exposure resulted in a significant changes in the activities of antioxidant enzymes, and induced hepatic lipoperoxidation. Moreover it resulted in histopathologic liver injury as well as significant increase of serum activity of AST and ALT.

• Authors: Luis Josino Brasil, José Luiz Gomes do Amaral, Claudio Galeano Zettler, Claudio Augusto Marroni, Rafael Vercelino, Norma Marroni
• Journal: Arquivos de gastroenterologia (Arq Gastroenterol) 2007 Jan-Mar Vol. 44 Issue 1 Pg. 73-7 ISSN: 0004-2803 [Print] Brazil
• Vernacular Title: Modelo experimental de indução de lesão oxidativa hepática em ratos por halotano.
• PMID: 17639188 (Publication Type: Journal Article)

Chemical References

• Anesthetics, Inhalation
• Aspartate Aminotransferases
• Alanine Transaminase
• Halothane
• Phenobarbital

Topics

• Alanine Transaminase (blood)
• Anesthetics, Inhalation (pharmacology)
• Animals
• Aspartate Aminotransferases (blood)
• Disease Models, Animal
• Halothane (pharmacology)
• Hypoxia (complications)
• Lipid Peroxidation (drug effects)
• Liver (drug effects, enzymology, pathology)
• Male
• Oxidative Stress (drug effects)
• Phenobarbital (pharmacology)
• Rats
• Rats, Wistar