[Modulation effect of naomaitong on gelatinase system after cerebral ischemia/reperfusion in rats LI].

Abstract

OBJECTIVE:

To study the modulation effect of Naomaitong on gelatinase system after cerebral ische-Focal cerebral I/R rat model was duplicated by method of the intralumimia/reperfusion (I/R) in rats.

METHOD:

nal filament technique. Rats were randomly divided into the sham-operative group, the model group, the Naomaitong group and the Nimodipine group, the latter three groups were also divided into the 3 hrs after ischemia group, and 6 hrs, 12 hrs, 24 hrs, 3 d, 6 d after IR groups. Immunohistochemical method and zymogram analysis method, etc. were adopted to observe the change of microvessel structure, gelatinase and its inhibitor expression.

RESULTS:

MMP-2 (IR 24 h-6 d)and MMP-9 (I/R 12 h-3 d) expression levels could be lowered and TIMP-1 expression level (IR 24 h-6 d) improved by Naomaitong. Besides, comparison of MMP-2 and MMP-9 content in zymogram analysis in each group showed that changes of its quantity were in accordance with the laws of immune expression.

CONCLUSION:

The protective effect of Naomaitong on cerebromicrovessel basement membrane injury in rats is related to its modulation on gelatinase system.

• Authors: Jian-sheng Li, Ke Liu, Ming-hang Wang
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 26 Suppl Pg. 14-7 (Jun 2006) ISSN: 1003-5370 [Print] China
• PMID: 17569336 (Publication Type: English Abstract, Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Protective Agents
• Tissue Inhibitor of Metalloproteinase-1
• naomaitong
• Gelatinases
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Animals
• Basement Membrane (drug effects, metabolism)
• Brain Ischemia (enzymology)
• Disease Models, Animal
• Drugs, Chinese Herbal (pharmacology)
• Gelatinases (metabolism)
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Medicine, Chinese Traditional
• Microvessels (drug effects, metabolism)
• Protective Agents (pharmacology)
• Rats
• Reperfusion Injury (enzymology)
• Tissue Inhibitor of Metalloproteinase-1 (metabolism)