Abstract | BACKGROUND: HYPOTHESIS: ANIMALS: Twenty-eight dogs with various stages of HSA were enrolled in the study. METHODS: The HSA vaccine was prepared with lysates of allogeneic canine HSA cell lines mixed with an adjuvant composed of liposome- DNA complexes. Dogs received a series of 8 immunizations administered over a 22-week period, and most also received chemotherapy. Clinical adverse effects were noted, immune responses were measured by enzyme-linked immunosorbent assay (ELISA) and flow cytometry, and survival times were calculated. RESULTS: The most common adverse effects observed in vaccinated dogs also treated with doxorubicin chemotherapy were diarrhea and anorexia. Vaccinated dogs were found to mount strong humoral immune responses against a control antigen and, most dogs also mounted antibody responses against canine HSA cells. Thirteen dogs with stage II splenic HSA that received the tumor vaccine plus doxorubicin chemotherapy had an overall median survival time of 182 days. CONCLUSIONS: We conclude that an allogeneic tumor lysate vaccine is safe in dogs with HSA and can elicit humoral immune responses in dogs that are receiving concurrent doxorubicin chemotherapy.
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Authors | Lance W U'Ren, Barbara J Biller, Robyn E Elmslie, Douglas H Thamm, Steven W Dow |
Journal | Journal of veterinary internal medicine
(J Vet Intern Med)
2007 Jan-Feb
Vol. 21
Issue 1
Pg. 113-20
ISSN: 0891-6640 [Print] United States |
PMID | 17338158
(Publication Type: Clinical Trial, Phase I, Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Antineoplastic Agents
- Cancer Vaccines
- Doxorubicin
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Topics |
- Animals
- Antineoplastic Agents
(therapeutic use)
- Cancer Vaccines
(adverse effects, therapeutic use)
- Chemotherapy, Adjuvant
(veterinary)
- Dog Diseases
(drug therapy)
- Dogs
- Doxorubicin
(therapeutic use)
- Drug Therapy, Combination
- Heart Neoplasms
(drug therapy, veterinary)
- Hemangiosarcoma
(drug therapy, veterinary)
- Kidney Neoplasms
(drug therapy, veterinary)
- Skin Neoplasms
(drug therapy, veterinary)
- Splenic Neoplasms
(drug therapy, veterinary)
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