Abstract |
The association between the clinical outcome of chloroquine treatment and mutations in the putative Plasmodium falciparum chloroquine resistance transporter (Pfcrt) gene at codon 76 and multidrug resistance gene 1 (Pf mdr1) at codon 86 were investigated among 406 children with uncomplicated malaria presenting at five sentinel health centres in Ghana. Presence of mutations in isolates taken at pre-treatment and on day of recurrence of parasites was detected using PCR followed by RFLP techniques. The prevalence of Pfcrt T76 mutants was 80% at Hohoe, 46% at Navrongo, 98% at Tarkwa, 61% at Sunyani and 46% at Yendi. The prevalence of the mutant Pfmdr1 at Hohoe, Navrongo, Tarkwa, Sunyani and Yendi were 78, 58, 95, 53 and 42%, respectively. Significant association between the Pfcrt mutation and treatment outcome was observed at Hohoe and Sunyani (p < 0.05), but not at Navrongo, Tarkwa or Yendi (p > 0.05). Similarly, a statistical significant association between Pfmdr1 86 and treatment failures was observed at Hohoe and Sunyani (p < 0.05) but not at the other three sites. A positive correlation was found between mutant Pfcrt prevalence only and treatment failures with a Spearman's rho-value of 0.872 and a p-value = 0.027. All parasite isolates from samples taken at recrudescence from patients with chloroquine treatment failures were found to have both Pfcrt and Pfmdr mutations.
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Authors | Nancy O Duah, Michael D Wilson, Anita Ghansah, Ben Abuaku, Dominic Edoh, Neils B Quashie, Kwadwo A Koram |
Journal | Journal of tropical pediatrics
(J Trop Pediatr)
Vol. 53
Issue 1
Pg. 27-31
(Feb 2007)
ISSN: 0142-6338 [Print] England |
PMID | 17158810
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ATP-Binding Cassette Transporters
- Antimalarials
- Codon
- Protozoan Proteins
- mdr gene protein, Plasmodium
- Chloroquine
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Topics |
- ATP-Binding Cassette Transporters
(genetics)
- Animals
- Antimalarials
(pharmacology)
- Chi-Square Distribution
- Child, Preschool
- Chloroquine
(pharmacology)
- Codon
- Drug Resistance, Multiple
- Female
- Genes, Protozoan
(genetics)
- Ghana
(epidemiology)
- Humans
- Infant
- Malaria, Falciparum
(drug therapy, genetics)
- Male
- Mutation
- Plasmodium falciparum
(genetics)
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Prevalence
- Protozoan Proteins
(genetics)
- Treatment Outcome
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