Up-regulation of interleukin-6 gene expression in cyclophosphamide-induced cystitis in mice: An in situ hybridization histochemical study.

Abstract

AIM:

To determine the time course and the cellular sources of interleukin (IL)-6 in the bladder during experimental cystitis, the expression of the IL-6 gene and IL-6 protein was examined in the bladder during cyclophosphamide (CP)-induced cystitis.

METHODS:

Mice were killed at 0, 1, 2, 6, 12 and 48 h after the intraperitoneal administration of 0.9% saline containing either CP (200 mg/kg) or saline. The expression of IL-6 gene and IL-6 protein were detected using in situ hybridization histochemistry and immunocytochemistry, respectively.

RESULTS:

In situ hybridization histochemistry showed that IL-6 gene expression was significantly up-regulated in the bladder at 1 h in comparison to that at 0 h after CP administration. The levels of IL-6 gene expression peaked at 6 h after CP administration and then declined thereafter. In contrast, only a few IL-6 transcripts were expressed in the bladder but they remained unchanged following the administration of saline at all time points examined. The IL-6 transcripts were predominantly distributed in the perivascular area of the submucosal layers during CP-induced cystitis. Immunocytochemistry demonstrated IL-6 immunoreactivity in the spindle-shaped cells located in the vicinity of the dilated vessels of the submucosal layers during CP-induced cystitis.

CONCLUSION:

IL-6 gene expression was up-regulated in the submucosal layer of the bladder and peaked at 6 h after CP administration, suggesting that the primary source of IL-6 may be fibroblasts in the bladder during CP-induced cystitis.

• Authors: Hisae Nishii, Masayoshi Nomura, Naohiro Fujimoto, Tetsuro Matsumoto
• Journal: International journal of urology : official journal of the Japanese Urological Association (Int J Urol) Vol. 13 Issue 10 Pg. 1339-43 (Oct 2006) ISSN: 0919-8172 [Print] Australia
• PMID: 17010015 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Interleukin-6
• RNA, Messenger
• Cyclophosphamide

Topics

• Animals
• Biomarkers (metabolism)
• Cyclophosphamide (toxicity)
• Cystitis (chemically induced, genetics, metabolism)
• Disease Models, Animal
• Immunohistochemistry
• In Situ Hybridization (methods)
• Interleukin-6 (biosynthesis, genetics)
• Male
• Mice
• RNA, Messenger (genetics)
• Up-Regulation
• Urinary Bladder (drug effects, metabolism, pathology)