Angiogenesis is characteristic of cartilage
tumors, not of normal cartilage tissue. In addition to our previous report on differential expression of proangiogenic
vascular endothelial growth factor A (
VEGF-A) in cartilage
tumors, we analyzed the expression of a
disintegrin and
metalloproteinase with
thrombospondin motifs 1 (ADAMTS1), which has been identified as a potent inhibitor of
VEGF-A. We further used a
chondrosarcoma cell line to study the effect of
interleukin (IL)-1beta and
hypoxia on the regulation of ADAMTS1 and
VEGF-A expression. ADAMTS1 was detected by
reverse transcriptase-polymerase chain reaction and immunohistochemistry in all analyzed samples from
enchondromas, conventional chondrosacromas, and dedifferentiated
chondrosarcomas without exception. In contrast to previous reports on other
cancers, we did not detect a consistent decrease in ADAMTS1 expression in
chondrosarcomas.
Interleukin-1beta stimulation, not
hypoxia, transcriptionally downregulated ADAMTS1 in
chondrosarcoma cells, whereas
VEGF-A expression was upregulated either by
hypoxia or IL-1beta. We conclude that ADAMTS1 and
VEGF-A in
chondrosarcoma cells are regulated independently from each other. We believe that IL-1beta has a stronger impact on vascularization in
chondrosarcomas than
hypoxia, as both factors, ADAMTS1 and
VEGF-A, are regulated in a way that favors angiogenesis.