Abstract | AIM: To investigate the ocular surface inflammatory response to chronic topical treatments in patients with glaucoma by measuring the cytokine level in tears using multiplex bead analysis. METHODS: Tear samples were collected from 21 patients with glaucoma and 12 healthy volunteers. Tears were analysed for the presence of 17 cytokines: interleukin (IL)1beta, IL2, IL4, IL5, IL6, IL7, IL8, IL10, IL12, IL13, IL17, granulocyte-colony stimulating factor, granulocyte-macrophage stimulating factor, interferon (INF)gamma, monocyte chemotactic protein (MCP)1, macrophage inflammatory protein 1beta and tumour necrosis factor ( TNF)alpha. The cytokines in each sample of tears were measured using multiplex bead analysis with microspheres as solid support for immunoassays. RESULTS: In the tears of treated patients, proinflammatory cytokines (IL1beta, IL6, IL12, TNFalpha) were significantly increased compared with controls. T helper (Th)1 (INFgamma, IL2) and Th2 ( IL5, IL10, IL4) type cytokines were also significantly higher (p<0.05); however, the most marked increase was observed with Th1 cytokines. The expression of chemokine IL8 and MCP1 was also increased in the treated group. CONCLUSION: This study shows that pro-inflammatory cytokine secretion by conjunctival cells is increased in response to topical treatments for glaucoma. The characterisation of cytokines in tears was previously limited by the small volume attainable, a limitation that has been overcome by multiplex analysis.
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Authors | L Malvitte, T Montange, A Vejux, C Baudouin, A M Bron, C Creuzot-Garcher, G Lizard |
Journal | The British journal of ophthalmology
(Br J Ophthalmol)
Vol. 91
Issue 1
Pg. 29-32
(Jan 2007)
ISSN: 0007-1161 [Print] England |
PMID | 16943231
(Publication Type: Journal Article)
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Chemical References |
- Amides
- Antihypertensive Agents
- Chemokines
- Cytokines
- Lipids
- Prostaglandins F, Synthetic
- Quinoxalines
- Sulfonamides
- Thiophenes
- Cloprostenol
- Brimonidine Tartrate
- Latanoprost
- Timolol
- Carteolol
- dorzolamide
- Bimatoprost
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Topics |
- Administration, Topical
- Aged
- Amides
(administration & dosage)
- Antihypertensive Agents
(administration & dosage)
- Bimatoprost
- Brimonidine Tartrate
- Carteolol
(administration & dosage)
- Case-Control Studies
- Chemokines
(analysis)
- Cloprostenol
(administration & dosage, analogs & derivatives)
- Cytokines
(analysis)
- Dose-Response Relationship, Immunologic
- Drug Therapy, Combination
- Female
- Glaucoma
(drug therapy, immunology)
- Humans
- Intraocular Pressure
(drug effects)
- Latanoprost
- Lipids
(administration & dosage)
- Male
- Prospective Studies
- Prostaglandins F, Synthetic
(administration & dosage)
- Quinoxalines
(administration & dosage)
- Sulfonamides
(administration & dosage)
- Tears
(immunology)
- Thiophenes
(administration & dosage)
- Timolol
(administration & dosage)
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