Abstract | OBJECTIVES: METHODS: RESULTS: TrkA mRNA levels were over-expressed in ovarian cancer compared to normal ovarian samples, whereas NGF mRNA levels remained unchanged. NGF and trkA proteins were absent or found in very low levels in normal ovarian surface epithelium (OSE), whereas they were highly expressed in epithelial cells of EOC. Additionally, NGF stimulated the expression of VEGF isoforms in cancer explants. The effect was dose-dependent and inhibited by a NGF antibody and by K(252a), a trk receptor inhibitor. CONCLUSION: The abundance of NGF and trkA receptors in epithelial cells of EOC, together with the ability of NGF to increase VEGF expression strongly suggests an autocrine role of NGF in EOC. These findings suggest that blocking neurotrophin action could be a therapeutic target in treating ovarian cancer.
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Authors | Ximena Campos, Yenny Muñoz, Alberto Selman, Roberto Yazigi, Leonor Moyano, Caroline Weinstein-Oppenheimer, Hernán E Lara, Carmen Romero |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 104
Issue 1
Pg. 168-75
(Jan 2007)
ISSN: 0090-8258 [Print] United States |
PMID | 16935322
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Vascular Endothelial Growth Factor A
- Nerve Growth Factor
- Receptor, trkA
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Topics |
- Adult
- Female
- Humans
- Immunohistochemistry
- Middle Aged
- Nerve Growth Factor
(biosynthesis, genetics, metabolism)
- Ovarian Neoplasms
(enzymology, genetics, metabolism)
- Ovary
(enzymology, metabolism)
- RNA, Messenger
(biosynthesis, genetics)
- Receptor, trkA
(biosynthesis, genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Vascular Endothelial Growth Factor A
(biosynthesis, genetics)
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