Abstract | BACKGROUND: METHODS: TRAP concentrations in the serum of tumor patients were determined by ELISA. The expression of TRAP in breast, ovarian, and cervical cancer and malignant melanoma was analyzed by immunohistochemistry. RT-PCR and immunocytology were used to evaluate TRAP expression in cultured tumor cells. RESULTS: A marked increase in serum TRAP concentrations was observed in patients with breast and ovarian cancer, regardless of the presence or absence of bone disease. TRAP expression was found in breast and ovarian cancers and malignant melanoma, while cervical cancer showed only minimal expression of TRAP. Expression of TRAP was absent in benign tissue or was much less marked than in the corresponding malignant tissue. TRAP expression was also demonstrated in cultured primary cancer cells and in commercially available cell lines. CONCLUSION: Overexpression of TRAP was detected in the cells of various different tumors. TRAP might be useful as a marker of progression of malignant disease. It could also be a potential target for future cancer therapies.
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Authors | A Honig, L Rieger, M Kapp, M Krockenberger, M Eck, J Dietl, U Kämmerer |
Journal | BMC cancer
(BMC Cancer)
Vol. 6
Pg. 199
(Jul 25 2006)
ISSN: 1471-2407 [Electronic] England |
PMID | 16869970
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Isoenzymes
- Acid Phosphatase
- Tartrate-Resistant Acid Phosphatase
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Topics |
- Acid Phosphatase
(blood, metabolism)
- Breast Neoplasms
(metabolism)
- Control Groups
- Enzyme-Linked Immunosorbent Assay
- Female
- Gene Expression
- Humans
- Immunohistochemistry
- Isoenzymes
(blood, metabolism)
- Melanoma
(metabolism)
- Ovarian Neoplasms
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Tartrate-Resistant Acid Phosphatase
- Tumor Cells, Cultured
- Up-Regulation
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