Association between ADAM33 polymorphisms and adult asthma in the Japanese population.

Abstract

BACKGROUND:

ADAM33, a member of the ADAM (a disintegrin and metalloprotease) family, is a putative asthma susceptibility gene recently identified by positional cloning. It is important to know whether the association exists in ethnically diverse populations.

OBJECTIVE:

To assess whether genetic functional variants of ADAM33 relate to the susceptibility or some phenotypes in adult patients with bronchial asthma in a Japanese population.

METHODS:

We searched for single nucleotide polymorphisms (SNPs) in ADAM33 by PCR-directed sequencing and identified 48 SNPs. Fourteen SNPs were selected with regard to the LD pattern, and genotyped by Taq-Man and PCR-RFLP methods. We conducted an association study of ADAM33 with 504 adult asthmatic patients and 651 controls, and haplotype analyses of related variants were performed.

RESULTS:

Significant associations with asthma were found for the SNPs T1 (Met764Thr), T2 (Pro774Ser), S2 and V-3 (with the lowest P-value for T1, P = 0.0015; OR 0.63). We analysed the haplotype using these four polymorphisms, and found a positive association with haplotype CCTG (P = 0.0024).

CONCLUSION:

Our results replicate associations reported recently in other ethnic populations, and suggest that the ADAM33 gene is involved in the development of asthma through genetic polymorphisms.

• Authors: T Hirota, K Hasegawa, K Obara, A Matsuda, M Akahoshi, K Nakashima, T Shirakawa, S Doi, K Fujita, Y Suzuki, Y Nakamura, M Tamari
• Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 36 Issue 7 Pg. 884-91 (Jul 2006) ISSN: 0954-7894 [Print] England
• PMID: 16839403 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Disintegrins
• ADAM Proteins
• ADAM33 protein, human

Topics

• ADAM Proteins (genetics)
• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Asian People (genetics)
• Asthma (ethnology, genetics)
• Case-Control Studies
• Disintegrins (genetics)
• Female
• Gene Frequency
• Haplotypes
• Humans
• Japan (epidemiology)
• Linkage Disequilibrium
• Male
• Middle Aged
• Phenotype
• Polymorphism, Single Nucleotide