The aim of this work is to study the effect of the
flavonoids rutin and
quercetin on hepatic
monooxygenase activities in experimental influenza virus
infection (EIVI). EIVI causes oxidative stress in the whole organism. This is confirmed by the rapidly increased concentrations of thiobarbituric reactive substances in
influenza-infected mice: lungs - 290%; blood plasma - more than 320%; liver - 230%; brain - 50%. Although known for their
antioxidant activities,
rutin and
quercetin exhibit prooxidant effect in healthy and
antioxidant activity in
influenza-infected animals. The pretreatment with both
flavonoids (20 mg/kg b.w.) restores oxidative damage mostly in the target organ of the
infection as well as in the liver of all infected mice (lungs:
rutin - 30%,
quercetin - 40%, combination - 45%; liver:
rutin - 12%;
quercetin - 40%; combination - 50%). As far as EIVI causes oxidative stress, toxicosis and inhibition of the hepatic
monooxygenase activity, it is important to study the effects of
rutin and
quercetin on these systems. Both
flavonoids induce the level of
cytochrome P-450 (
rutin - 13%,
quercetin - 30%, combination - 22%) but inactivate
NADPH-cytochrome c reductase,
aminopyrine N-demethylase and
analgin N-demethylase on the 5th day of EIVI. Probably, these
flavonoids affect different components of the
monooxygenase system. These effects could be explained with oxidative hepatic intoxication on the 5th critical day of EIVI as well as higher dose treatment. More data are needed on the
antioxidant/prooxidant effects of
rutin and
quercetin, probably due to specific metabolic and physiological activities, chemical structure, etc.