Central memory CD4 T cells are the predominant cell subset resistant to anergy in SIV disease resistant sooty mangabeys.

Abstract

OBJECTIVE:

HIV infection in humans and experimental SIV infection in rhesus macaques leads to the loss of recall antigen responses, immunological anergy in CD4 T cells and AIDS. In contrast, natural infection in sooty mangabeys with SIV does not lead to disease despite high viral loads accompanied by resistance of their CD4 T cells to undergo immunological anergy. The objective of the study was to further elucidate the mechanisms that contribute to anergy resistance in CD4 T cells from sooty mangabeys and identify whether the anergy resistance is a function of a specific subset or the entire cell population.

MATERIALS AND METHODS:

Susceptibility or resistance to anergy was analyzed in antigen specific and defined CD4 T cell subsets from peripheral blood of sooty mangabeys and rhesus macaques by using an in vitro anergy inducing protocol; expression of the anergy associated gene GRAIL was measured.

RESULTS:

Antigen specific CD4 T cells from SIV disease resistant sooty mangabey are relatively resistant to the development of anergy. This resistance is associated with a lack of an upregulation of GRAIL. Conversely, rhesus macaque CD4 T cells are sensitive to anergy induction associated with upregulation of GRAIL. Furthermore the anergy resistant phenotype of sooty mangabey CD4 T cells predominantly resides in central memory cells defined either as CD4+CD45RA-CD62L+ or CD4+CD28+CD95+CCR7+.

CONCLUSIONS:

The maintenance of recall responses in sooty mangabeys is associated with the resistance of central memory CD4 T cells to the induction of anergy which may represent an important mechanism underlying SIV disease resistance in this species.

• Authors: Pavel Bostik, Erika S Noble, Ann E Mayne, Lisa Gargano, Francois Villinger, Aftab A Ansari
• Journal: AIDS (London, England) (AIDS) Vol. 20 Issue 2 Pg. 181-8 (Jan 09 2006) ISSN: 0269-9370 [Print] England
• PMID: 16511410 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Hemocyanins
• Ubiquitin-Protein Ligases
• keyhole-limpet hemocyanin

Topics

• Animals
• CD4-Positive T-Lymphocytes (immunology, metabolism)
• Cells, Cultured
• Cercocebus atys (immunology, virology)
• Clonal Anergy (immunology)
• Hemocyanins (immunology)
• Humans
• Immunity, Innate
• Immunologic Memory
• Immunophenotyping
• Lymphocyte Activation (immunology)
• Macaca mulatta (immunology)
• Monkey Diseases (immunology)
• Simian Acquired Immunodeficiency Syndrome (immunology)
• Species Specificity
• T-Lymphocyte Subsets (immunology)
• Ubiquitin-Protein Ligases (metabolism)