Time course of oxidative stress status in the postprandial and postabsorptive states in type 1 diabetes mellitus: relationship to glucose and lipid changes.

Abstract

OBJECTIVE:

The aim of this study was to compare oxidative stress status (OSS) with blood glucose and lipid changes during the fasting, postprandial and postabsorptive phases in type 1 diabetes mellitus.

METHODS:

Twenty-three patients on intensive insulin treatment received a standard fat-rich breakfast and lunch. OSS was monitored at fasting (F), just after the post-breakfast glycemia peak (BP) (identified by continuous subcutaneous glucose monitoring), 3.5-h post-breakfast (B3.5), just after the post-lunch peak (LP), just after the post-lunch dale (LD) and 5 hours after lunch (L5).

RESULTS:

Whereas whole blood glutathione and plasma protein thiols increased in the postprandial period (from 6.52 +/- 1.20 (F) to 7.08 +/- 1.45 micromol/g Hb (BP), p = 0.005), ascorbate decreased gradually from 44 +/- 17 (F) to 39 +/- 19 micromol/L (LD), p = 0.015. Retinol and alpha-tocopherol also decreased from 27.1 +/- 7.0 (F) to 25.3 +/- 5.2 micromol/L (BP), p = 0.005. Uric acid decreased later, from 213 +/- 77 (BP) to 204 +/- 68 micromol/L (B3.5), p = 0.01, but then increased in LP (231 +/- 70 micromol/L) and LD to values higher than F (215 +/- 64, micromol/L, p = 0.01). Malondialdehyde increased gradually from 1.02 +/- 0.36 (F) to a maximum of 1.14 +/- 0.40 micromol/L (LP). In the postabsorptive phase (L5) all parameters except for thiols reverted to fasting concentrations.

CONCLUSIONS:

In type 1 diabetes lipid peroxidation increases during the postprandial phase in parallel to glucose and triglyceride changes. Blood antioxidants, however, followed diverse patterns of change.

• Authors: Begoña Manuel-y-Keenoy, Ann Van Campenhout, Petra Aerts, Jan Vertommen, Pascale Abrams, Luc F Van Gaal, Carolien Van Gils, Ivo H De Leeuw
• Journal: Journal of the American College of Nutrition (J Am Coll Nutr) Vol. 24 Issue 6 Pg. 474-85 (Dec 2005) ISSN: 0731-5724 [Print] United States
• PMID: 16373944 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Blood Glucose
• Insulin
• Lipids
• Triglycerides
• Glutathione

Topics

• Absorption
• Adult
• Antioxidants (metabolism)
• Area Under Curve
• Blood Glucose (analysis)
• Diabetes Mellitus, Type 1 (blood)
• Fasting (blood)
• Female
• Food
• Glutathione (blood)
• Humans
• Insulin (metabolism)
• Lipid Peroxidation
• Lipids (blood)
• Male
• Middle Aged
• Oxidative Stress (physiology)
• Postprandial Period
• Triglycerides (blood)