Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell.

Abstract

Ganoderma lucidum Polysaccharide Peptide (Gl-PP) has shown some effects as anti-tumors in mice and potential anti-angiogenesis. In this study, we elucidated the possible mechanism of Gl-PP action on anti-angiogenesis of tumor. Our research indicated that the proliferation of HUVECs was inhibited by Gl-PP in a dose-dependent fashion, but not because of cytotoxicity. Flow cytometric studies revealed that Gl-PP treatment of HUVECs could induce cell apoptosis directly. Moreover, addition of Gl-PP also led to a reduction of Bcl-2 anti-apoptotic protein expression and an increase of Bax pro-apoptotic protein expression of HUVECs. Therefore, inducing cell apoptosis by Gl-PP might be the mechanism of inhibiting HUVEC proliferation. Human lung carcinoma cells PG when exposed to high dose of Gl-PP in hypoxia for 18 h resulted in a decrease in the secreted VEGF. Taken together, these findings support the hypothesis that the key attribute of the anti-angiogenic potential of Gl-PP is that it may directly inhibit vascular endothelial cell proliferation or indirectly decrease growth factor expression of tumor cells.

• Authors: Qi-zhen Cao, Zhi-Bin Lin
• Journal: Life sciences (Life Sci) Vol. 78 Issue 13 Pg. 1457-63 (Feb 23 2006) ISSN: 0024-3205 [Print] Netherlands
• PMID: 16269156 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Polysaccharides
• Vascular Endothelial Growth Factor A

Topics

• Apoptosis (drug effects)
• Cell Division (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Drugs, Chinese Herbal (pharmacology)
• Endothelium, Vascular (cytology, drug effects)
• Flow Cytometry
• Ganoderma
• Humans
• Lung Neoplasms
• Polysaccharides (pharmacology)
• Reishi
• Tumor Cells, Cultured
• Umbilical Veins
• Vascular Endothelial Growth Factor A (metabolism)