Propofol attenuates Kupffer cell activation during hypoxia-reoxygenation.

Abstract

PURPOSE:

We undertook a study to determine whether propofol may attenuate Kupffer cell (KC) activation, thus protecting the cells against hypoxia-reoxygenation injury through the modulation of intracellular calcium ([Ca2+]i).

METHODS:

[Ca2+]i, the expression of tumour necrosis factor (TNF)-alpha mRNA, and KC viability were measured in response to hypoxia-reoxygenation following pretreatment with propofol 0.5 and 5 microg.mL(-1) (Groups P1 and P2, respectively) or without propofol (Group HRC). KCs were isolated and cultured from male Sprague-Dawley rats. KCs were incubated under an atmosphere of hypoxia (95% N2 + 5% CO2) for 60 min with subsequent 120 min reoxygenation (95% air + 5% CO2). [Ca2+]i for the first 12 min after reoxygenation, TNF-alpha mRNA, and KC viability at the end of reoxygenation in groups P1 and P2 were compared with those of HRC.

RESULTS:

The increase of [Ca2+]i from the baseline was attenuated in P1 (96.6 +/- 6.9%) and P2 (96.1 +/- 5.4%) compared with HRC (143.8 +/- 11.5%), (P < 0.001), with no difference between P1 and P2. The expression of TNF-alpha mRNA increased only in HRC during hypoxia-reoxygenation. KC viability increased in P1 (97.5 +/- 2.6%) and P2 (94.6 +/- 2.9%), compared with HRC (89.9 +/- 1.4%), (P < 0.005), with no difference between P1 and P2.

CONCLUSION:

The results indicate that propofol attenuates KC activation and protects KC from hypoxia-reoxygenation injury at clinically relevant concentrations. This attenuation seems to result from inhibition of [Ca2+]i increase in KC.

• Authors: Eon-Gi Sung, Daelim Jee, In-Hwan Song, Hee-Sun Kim, Jae Hoon Bae, Se-Hun Park
• Journal: Canadian journal of anaesthesia = Journal canadien d'anesthesie (Can J Anaesth) Vol. 52 Issue 9 Pg. 921-6 (Nov 2005) ISSN: 0832-610X [Print] United States
• PMID: 16251556 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anesthetics, Intravenous
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• Oxygen
• Calcium
• Propofol

Topics

• Anesthetics, Intravenous (pharmacology)
• Animals
• Calcium (metabolism)
• Cell Separation
• Cell Survival (drug effects)
• Cells, Cultured
• Hypoxia (physiopathology)
• Kupffer Cells (drug effects)
• Male
• Oxygen (blood)
• Propofol (pharmacology)
• RNA, Messenger (biosynthesis)
• Rats
• Rats, Sprague-Dawley
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Necrosis Factor-alpha (biosynthesis)