Correlation between laminin-5 gamma2 chain and epidermal growth factor receptor expression in esophageal squamous cell carcinomas.

Abstract

OBJECTIVES:

Laminin-5 gamma2 chain (LN-5 gamma2) is an extracellular matrix protein that plays an important role in cell migration and tumor invasion. We evaluated the association of LN-5 gamma2 and epidermal growth factor receptor (EGFR) expression in esophageal squamous cell carcinoma (SCC).

METHODS:

LN-5 gamma2 and EGFR expression was evaluated in 110 esophageal SCC patients by immunohistochemistry, and was confirmed using esophageal SCC cell lines by Western blot analysis.

RESULTS:

LN-5 gamma2 expression in the invasive front of the tumor was correlated with the depth of invasion (p = 0.0001), lymph node metastasis (p = 0.0011) and pathological stage (p = 0.0001). The strong expression of EGFR was also correlated with lymph node metastasis (p = 0.0456) and the pathological stage (p = 0.0055). In patient survival, LN-5 gamma2 positivity and/or strong EGFR expression showed a significantly low survival rate as compared with those with lesser expression of LN-5 gamma2 and EGFR. Immunohistochemically, LN-5 gamma2 expression was significantly correlated with EGFR expression (p < 0.0001). Western blot analysis also confirmed the correlated expression of LN-5 gamma2 and EGFR in SCC cell lines except 2 of the 5 cell lines.

CONCLUSIONS:

This study suggests that coexpression of LN-5 gamma2 and EGFR is closely related to the progression and poor prognosis of esophageal SCC.

• Authors: Yasuyuki Fukai, Norihiro Masuda, Hiroyuki Kato, Minoru Fukuchi, Tatsuya Miyazaki, Masanobu Nakajima, Makoto Sohda, Hiroyuki Kuwano, Takashi Nakajima
• Journal: Oncology (Oncology) Vol. 69 Issue 1 Pg. 71-80 ( 2005) ISSN: 0030-2414 [Print] Switzerland
• PMID: 16103736 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Cell Adhesion Molecules
• ErbB Receptors

Topics

• Aged
• Biomarkers, Tumor (analysis)
• Blotting, Western
• Carcinoma, Squamous Cell (chemistry, pathology, surgery)
• Cell Adhesion Molecules (analysis)
• ErbB Receptors (analysis)
• Esophageal Neoplasms (chemistry, pathology, surgery)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Neoplasm Staging
• Prognosis
• Survival Analysis
• Kalinin